Characterization of vasoactive intestinal peptide (VIP) receptors in mammalian lung.

Dickinson KE, Schachter M, Miles CM, Coy DH, Sever PS

Peptides1 Sept 1986Cited by 48 (Europe PMC)

PMID3025822DOI10.1016/0196-9781(86)90097-5PepBankPUB-03025822

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

4 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cell-free)Activity measurement
125I-VIP bound specifically to human, rat, guinea pig and rabbit lung membranes with a high-affinity dissociation constant of 60-200 pM.
In plain wordsIn lab tests, labelled VIP bound tightly and specifically to lung membranes from four species.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicable125I-VIP binding affinity (KD)
Study
human, rat, guinea pig and rabbitn: not reported in source
lung membrane preparations
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Measurements
KD
60 to 200 pM
lung membranes
Source sentences
Summary
125I-VIP bound specifically to sites on human, rat, guinea pig, and rabbit lung membranes with a dissociation constant (KD) of 60-200 pM and binding site maxima of 200-800 fmol/mg of protein.
Report a problemFND-0302582201
02LabIn vitro (cell-free)Activity measurement
Lung membranes from four species showed 125I-VIP binding site maxima of 200-800 fmol/mg of protein.
In plain wordsIn lab tests, lung membranes from four species held a measurable number of VIP binding sites.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicable125I-VIP binding site maxima
Study
human, rat, guinea pig and rabbitn: not reported in source
lung membrane preparations
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Measurements
Binding site maxima
200 to 800 fmol/mg protein
lung membranes
Source sentences
Summary
125I-VIP bound specifically to sites on human, rat, guinea pig, and rabbit lung membranes with a dissociation constant (KD) of 60-200 pM and binding site maxima of 200-800 fmol/mg of protein.
Report a problemFND-0302582202
03LabIn vitro (cell-free)Activity measurement
A second, lower-affinity VIP binding site was detected in lung membranes but not investigated further.
In plain wordsIn lab tests, a second weaker VIP binding site was seen in lung membranes.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableSecond lower-affinity VIP binding site
Study
human, rat, guinea pig and rabbitn: not reported in source
lung membrane preparations
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
The presence of a second lower affinity site was detected but not investigated further.
Report a problemFND-0302582203
04LabIn vitro (cell-free)Activity measurement
High-affinity 125I-VIP binding was reversible and displaced by related peptides in the order VIP greater than rGRF greater than PHI greater than hGRF greater than secretin.
In plain wordsIn lab tests, VIP binding to lung membranes was reversible, and VIP itself displaced the label best among the peptides tested.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableDisplacement of 125I-VIP by related peptides
Study
human, rat, guinea pig and rabbitn: not reported in source
lung membrane preparations
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
High affinity 125I-VIP binding was reversible and displaced by structurally related peptides with an order of potency: VIP greater than rGRF greater than PHI greater than hGRF greater than secretin = Ac Tyr1 D Phe2 GRF.
Report a problemFND-0302582204

Studies that did not test the peptide alone4

In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).

05LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cell-free)Other
Covalently labelled human, rat and rabbit lung membranes showed major VIP-receptor complexes of about 65,000, 56,000 and 64,000 daltons.
In plain wordsIn lab tests, the VIP receptor in lung membranes from three species had a size of about 56,000 to 65,000 daltons.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableSize of 125I-VIP-receptor complexes in human, rat and rabbit lung
Study
human, rat and rabbitn: not reported in source
lung membrane preparations
Outcome group
Receptor activity and mechanism · Pharmacology
Measurements
Mr
65,000 daltons
human lung
Mr
56,000 daltons
rat lung
Mr
64,000 daltons
rabbit lung
Source sentences
Summary
Sodium dodecyl sulfate-polyacrilamide gel electrophoresis of labeled human, rat, and rabbit lung membranes revealed major 125I-VIP-receptor complexes of: Mr = 65,000, 56,000, and 64,000 daltons, respectively.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0302582205
06LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cell-free)Other
Guinea pig lung membranes showed two 125I-VIP-receptor complexes of about 66,000 and 60,000 daltons.
In plain wordsIn lab tests, guinea pig lung membranes showed two VIP-receptor forms of different size.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableSize of 125I-VIP-receptor complexes in guinea pig lung
Study
guinea pign: not reported in source
lung membrane preparations
Outcome group
Receptor activity and mechanism · Pharmacology
Measurements
Mr
66,000 daltons
guinea pig lung
Mr
60,000 daltons
guinea pig lung
Source sentences
Summary
Guinea pig lung membranes exhibited two 125I-VIP-receptor complexes of Mr = 66,000 and 60,000 daltons.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0302582206
07LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cell-free)Other
Neuraminidase treatment of labelled lung VIP receptor complexes gave a single homogeneous band of about 57,000 daltons.
In plain wordsIn lab tests, removing sialic acid left one uniform VIP-receptor band, which suggests the receptor forms differ in sugar content.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableNeuraminidase treatment of labelled receptor
Study
human, rat, rabbit and guinea pign: not reported in source
lung membrane preparations
Outcome group
Receptor activity and mechanism · Pharmacology
Measurements
Mr
57,000 daltons
after neuraminidase
Source sentences
Summary
This labeling pattern probably reflects the presence of differentially glycosylated forms of the same receptor since treatment with neuroaminidase resulted in a single homogeneous band (Mr = 57,000 daltons).
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0302582207
08LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cell-free)Other
Soluble covalently labelled VIP receptors from guinea pig and human lung bound to wheat germ agglutinin columns and were specifically eluted.
In plain wordsIn lab tests, VIP receptors from guinea pig and human lung stuck to a sugar-binding column and could be released from it.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableBinding of solubilized VIP receptors to wheat germ agglutinin
Study
guinea pig and humann: not reported in source
lung membrane preparations
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
Soluble covalently labeled VIP receptors from guinea pig and human lung bound to and were specifically eluted from agarose-linked wheat germ agglutinin columns.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0302582208

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
12 Oct 2026, 12:58 UTC
Study results updated
Version 1
9 Oct 2026, 12:41 UTC
First added · source update from Europe PMC

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