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Study · Ex vivo tissue · rat · 1989

3 results

Delta-sleep-inducing peptide (DSIP) stimulates the release of immunoreactive Met-enkephalin from rat lower brainstem slices in vitro.

Nakamura A, Nakashima M, Sakai K, Niwa M, Nozaki M, Shiomi H

Brain research1 Feb 1989Cited by 8 (Europe PMC)

PMID2706459 (opens in a new tab)DOI10.1016/0006-8993(89)90498-8 (opens in a new tab)PepBankPUB-02706459
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueMechanism

    In superfused rat lower brainstem slices, DSIP at 1 pM-1 nM significantly increased release of immunoreactive Met-enkephalin.

    In plain wordsIn lab tests on rat brain slices, DSIP made the tissue release more of the natural opioid Met-enkephalin.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    IncreaseMet-enkephalin release from brainstem slices
    Study

    ratn: not reported in source

    Superfused rat lower brainstem slices
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    1 pM-1 nM · superfusion in vitroAs given in this study (rat).

    Source sentences

    Summary
    DSIP at doses of 1 pM-1 nM significantly stimulated the release of immunoreactive Met-enkephalin (iME) from superfused slices of the rat lower brainstem.
    1 context sentence
    Summary · context
    We studied whether delta-sleep-inducing peptide (DSIP) acted on opioid receptor directly or indirectly.
  2. 02LabEx vivo tissueMechanism

    The DSIP-induced release of immunoreactive Met-enkephalin from rat brainstem slices depended on calcium.

    In plain wordsIn lab tests on rat brain slices, the Met-enkephalin release caused by DSIP needed calcium.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableCalcium dependence of DSIP-induced Met-enkephalin release
    Study

    ratn: not reported in source

    Superfused rat lower brainstem slices
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The DSIP-induced release of iME was calcium-dependent.
    1 context sentence
    Summary · context
    We studied whether delta-sleep-inducing peptide (DSIP) acted on opioid receptor directly or indirectly.
  3. 03LabIn vitro (cell-free)Mechanism

    DSIP showed no binding activity to any opioid receptor subtype.

    In plain wordsIn lab tests, DSIP did not bind to any type of opioid receptor.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    No significant changeOpioid receptor binding
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    DSIP did not have binding activity to any subtype of opioid receptors.
    1 context sentence
    Summary · context
    We studied whether delta-sleep-inducing peptide (DSIP) acted on opioid receptor directly or indirectly.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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