Oxytocin and progesterone release from bovine corpus luteal cells in culture: effects of insulin-like growth factor I, insulin, and prostaglandins.

McArdle CA, Holtorf AP

Endocrinology1 Mar 1989Cited by 48 (Europe PMC)

PMID2645114DOI10.1210/endo-124-3-1278PepBankPUB-02645114

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Natural levels measured4

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01LabEndogenous levelIn vitro (cells)Other
In cultured early bovine corpus luteum cells, IGF-I and insulin increased oxytocin release about 5-fold.
In plain wordsIn cow ovary cells in the lab, IGF-I and insulin raised the cells' own oxytocin release about fivefold.
Peptide
Oxytocin
Outcome
IncreaseOxytocin release after IGF-I or insulin
Study
bovinen: not reported in source
Primary early bovine corpus luteum cells in serum-free culture
Comparator
Untreated cells
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
Oxytocin release increase
5 fold
maximally effective IGF-I and insulin
EC50
0.27 nM
IGF-I
EC50
1.94 nM
insulin
Source sentences
Summary
Hormone release (measured between 60 and 84 h of culture) was increased approximately 5-fold (oxytocin) and 2.5-fold (progesterone) by maximally effective concentrations of IGF-I (EC50, 0.27 nM) and insulin (EC50, 1.94 nM).
2 context sentences(design, population, supporting numbers)
Summary · context
The release of oxytocin and progesterone from these cells was increased by the addition of insulin or insulin-like growth factor I (IGF-I), but not by IGF-II.
Summary · context
In the present work we describe a method for the preparation of cells from the early bovine corpus luteum (1-5 days postovulation) and their maintenance in serum-free culture.
This result measures the body's own Oxytocin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0264511401
02LabEndogenous levelIn vitro (cells)Other
In cultured early bovine corpus luteum cells, IGF-II did not increase oxytocin release.
In plain wordsIn cow ovary cells in the lab, IGF-II did not raise the cells' own oxytocin release.
Peptide
Oxytocin
Outcome
No significant changeOxytocin release after IGF-II
Study
bovinen: not reported in source
Primary early bovine corpus luteum cells in serum-free culture
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
The release of oxytocin and progesterone from these cells was increased by the addition of insulin or insulin-like growth factor I (IGF-I), but not by IGF-II.
1 context sentence(design, population, supporting numbers)
Summary · context
In the present work we describe a method for the preparation of cells from the early bovine corpus luteum (1-5 days postovulation) and their maintenance in serum-free culture.
This result measures the body's own Oxytocin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0264511402
03LabEndogenous levelIn vitro (cells)Other
In cultured bovine luteal cells, sustained prostaglandin exposure reduced oxytocin release in a dose-dependent way in the presence of IGF-I.
In plain wordsIn cow ovary cells in the lab, prostaglandins lowered the cells' own oxytocin release when IGF-I was present.
Peptide
Oxytocin
Outcome
DecreaseOxytocin release after prostaglandins
Study
bovinen: not reported in source
Primary early bovine corpus luteum cells in serum-free culture
Comparator
IGF-I alone
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
EC50
31 nM
PGF2 alpha
Source sentences
Summary
Sustained exposure (0-84 h) to prostaglandins (PGs) caused a dose-dependent reduction in oxytocin release in the presence of IGF-I (PGF2 alpha EC50, 31 nM; rank order of potency, PGF2 alpha greater than PGE2 greater than PGE1), but did not markedly reduce progesterone release.
1 context sentence(design, population, supporting numbers)
Summary · context
In the present work we describe a method for the preparation of cells from the early bovine corpus luteum (1-5 days postovulation) and their maintenance in serum-free culture.
This result measures the body's own Oxytocin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0264511403
04LabEndogenous levelIn vitro (cells)Other
In cultured bovine luteal cells, a protein kinase-C activator mimicked the inhibitory effect of prostaglandins on oxytocin production.
In plain wordsIn cow ovary cells in the lab, a protein kinase-C activator lowered the cells' own oxytocin output like prostaglandins did.
Peptide
Oxytocin
Outcome
DecreaseOxytocin release after protein kinase-C activator
Study
bovinen: not reported in source
Primary early bovine corpus luteum cells in serum-free culture
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
The inhibitory effect of PG on oxytocin production was mimicked by sustained exposure to a protein kinase-C activator (phorbol 12,13-dibutyrate), supporting the proposed role for this enzyme as a mediator of PG action.
1 context sentence(design, population, supporting numbers)
Summary · context
In the present work we describe a method for the preparation of cells from the early bovine corpus luteum (1-5 days postovulation) and their maintenance in serum-free culture.
This result measures the body's own Oxytocin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0264511404

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
11 Oct 2026, 20:03 UTC
Study results updated
Version 1
9 Oct 2026, 02:52 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.