Vasoactive intestinal polypeptide-induced chloride secretion by a colonic epithelial cell line. Direct participation of a basolaterally localized Na+,K+,Cl- cotransport system.

Dharmsathaphorn K, Mandel KG, Masui H, McRoberts JA

The Journal of clinical investigation1 Feb 1985Cited by 146 (Europe PMC)

PMID2579099PMCIDPMC423520DOI10.1172/jci111721PepBankPUB-02579099

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

2 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In human T84 colonic cell monolayers, VIP increased net chloride secretion, which accounted for the increase in short circuit current.
In plain wordsIn lab tests on human colon cells, VIP increased chloride secretion.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreaseNet chloride secretion and short circuit current
Study
humann: not reported in source
T84 colonic epithelial cell monolayers
Outcome group
Electrolyte balance · Gastrointestinal
Studied dose
Dose not stated
Source sentences
Summary
In a manner analogous to isolated intestine, the addition of VIP caused an increase of net Cl- secretion which accounted for the increase in short circuit current (Isc).
1 context sentence(design, population, supporting numbers)
Summary · context
We have used a well-differentiated human colonic cell line, the T84 cell line, as a model system to study the pathways of cellular ion transport involved in vasoactive intestinal polypeptide (VIP)-induced chloride secretion.
Report a problemFND-0257909901
02LabIn vitro (cells)Activity measurement
In T84 cells, the effect of VIP on short circuit current was dose dependent, with a threshold at 10(-10) M and a maximal effect at 10(-8) M.
In plain wordsIn lab tests on human colon cells, the VIP effect grew with the dose, from a low threshold to a maximum.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreaseDose dependence of the short circuit current response
Study
humann: not reported in source
T84 colonic epithelial cell monolayers
Outcome group
Electrolyte balance · Gastrointestinal
Studied dose
10(-10) M to 10(-8) MAs given in this study (human).
Source sentences
Summary
The effect of VIP on Isc was dose dependent with a threshold stimulation at 10(-10) M VIP, and a maximal effect at 10(-8) M.
1 context sentence(design, population, supporting numbers)
Summary · context
We have used a well-differentiated human colonic cell line, the T84 cell line, as a model system to study the pathways of cellular ion transport involved in vasoactive intestinal polypeptide (VIP)-induced chloride secretion.
Report a problemFND-0257909902

Studies that did not test the peptide alone3

In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).

03LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cells)Mechanism
In T84 cells, bumetanide prevented or reversed the response to VIP.
In plain wordsIn lab tests on human colon cells, the drug bumetanide stopped or reversed the response to VIP.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
DecreaseBumetanide inhibition of VIP response
Study
humann: not reported in source
T84 colonic epithelial cell monolayers
Outcome group
Electrolyte balance · Gastrointestinal
Source sentences
Summary
Bumetanide prevented or reversed the response to VIP.
1 context sentence(design, population, supporting numbers)
Summary · context
We have used a well-differentiated human colonic cell line, the T84 cell line, as a model system to study the pathways of cellular ion transport involved in vasoactive intestinal polypeptide (VIP)-induced chloride secretion.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0257909903
04LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cells)Mechanism
In T84 cells, bumetanide inhibited the VIP response promptly when added to the serosal medium, but not when added to the mucosal medium.
In plain wordsIn lab tests on human colon cells, bumetanide blocked the VIP response only when added to the serosal side.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
DecreaseBumetanide side of application
Study
humann: not reported in source
T84 colonic epithelial cell monolayers
Outcome group
Electrolyte balance · Gastrointestinal
Source sentences
Summary
Inhibition by bumetanide occurred promptly when it was added to the serosal, but not to the mucosal bathing media.
1 context sentence(design, population, supporting numbers)
Summary · context
We have used a well-differentiated human colonic cell line, the T84 cell line, as a model system to study the pathways of cellular ion transport involved in vasoactive intestinal polypeptide (VIP)-induced chloride secretion.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0257909904
05LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cells)Mechanism
In T84 cells, the response to VIP required the simultaneous presence of Na+, K+ and Cl- in the serosal medium.
In plain wordsIn lab tests on human colon cells, the VIP response needed sodium, potassium and chloride together on the serosal side.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableIon requirement of the VIP response
Study
humann: not reported in source
T84 colonic epithelial cell monolayers
Outcome group
Electrolyte balance · Gastrointestinal
Source sentences
Summary
Ion replacement studies demonstrated that the response to VIP required the simultaneous presence of Na+, K+, and Cl- in the serosal media.
1 context sentence(design, population, supporting numbers)
Summary · context
We have used a well-differentiated human colonic cell line, the T84 cell line, as a model system to study the pathways of cellular ion transport involved in vasoactive intestinal polypeptide (VIP)-induced chloride secretion.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0257909905

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
12 Oct 2026, 12:58 UTC
Study results updated
Version 1
9 Oct 2026, 12:41 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

MeSH terms

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