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Study · In vitro (cells) · 1989

7 results

In-vitro characterization of blood-brain barrier permeability to delta sleep-inducing peptide.

Raeissi S, Audus KL

The Journal of pharmacy and pharmacology1 Dec 1989Cited by 16 (Europe PMC)

PMID2576448 (opens in a new tab)DOI10.1111/j.2042-7158.1989.tb06385.x (opens in a new tab)PepBankPUB-02576448
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Pharmacokinetics

    In brain microvessel endothelial cell monolayers, DSIP flux was linear with time and increasing concentration (non-saturable).

    In plain wordsIn lab-grown brain barrier cells, DSIP crossed at a steady rate that did not level off as the concentration rose.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableTransendothelial flux versus time and concentration
    Study

    n: not reported in source

    Primary cultures of brain microvessel endothelial cell (BMEC) monolayers in side-by-side diffusion apparatus
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The transendothelial flux of the peptide was linear with time and increasing concentrations of DSIP (non-saturable)
    1 context sentence
    Summary · context
    The diffusion of delta sleep-inducing peptide (DSIP) across the blood-brain barrier (BBB) has been investigated with an in-vitro model comprised of primary cultures of brain microvessel endothelial cell (BMEC) monolayers.
  2. 02LabIn vitro (cells)Pharmacokinetics

    In brain microvessel endothelial cell monolayers, reduced temperature did not alter DSIP flux.

    In plain wordsIn lab-grown brain barrier cells, lowering the temperature did not change how much DSIP crossed.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    No significant changeEffect of reduced temperature on DSIP flux
    Study

    n: not reported in source

    Primary cultures of brain microvessel endothelial cell (BMEC) monolayers in side-by-side diffusion apparatus
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    but was not altered by reduced temperature.
    1 context sentence
    Summary · context
    The diffusion of delta sleep-inducing peptide (DSIP) across the blood-brain barrier (BBB) has been investigated with an in-vitro model comprised of primary cultures of brain microvessel endothelial cell (BMEC) monolayers.
  3. 03LabIn vitro (cells)Pharmacokinetics

    In brain microvessel endothelial cell monolayers, the apparent permeability coefficient for DSIP was in a range similar to water-soluble substances that cross the blood-brain barrier to a limited degree.

    In plain wordsIn lab-grown brain barrier cells, DSIP crossed about as easily as other small water-soluble substances that cross only a little.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableApparent permeability coefficient
    Study

    n: not reported in source

    Primary cultures of brain microvessel endothelial cell (BMEC) monolayers in side-by-side diffusion apparatus
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The apparent permeability coefficient for DSIP penetration of BMEC monolayers was in a range similar to water-soluble substances (e.g. fluorescein, fluorescein isothiocyanate dextrans) that penetrate the blood-brain barrier to a limited degree based on molecular weight.
    1 context sentence
    Summary · context
    The diffusion of delta sleep-inducing peptide (DSIP) across the blood-brain barrier (BBB) has been investigated with an in-vitro model comprised of primary cultures of brain microvessel endothelial cell (BMEC) monolayers.
  4. 04LabIn vitro (cells)Pharmacokinetics

    In brain microvessel endothelial cell monolayers, DSIP flux was bidirectional.

    In plain wordsIn lab-grown brain barrier cells, DSIP crossed in both directions.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableDirection of DSIP flux
    Study

    n: not reported in source

    Primary cultures of brain microvessel endothelial cell (BMEC) monolayers in side-by-side diffusion apparatus
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    DSIP flux across the BMEC monolayers was also found to be bidirectional
    1 context sentence
    Summary · context
    The diffusion of delta sleep-inducing peptide (DSIP) across the blood-brain barrier (BBB) has been investigated with an in-vitro model comprised of primary cultures of brain microvessel endothelial cell (BMEC) monolayers.
  5. 05LabIn vitro (cells)Pharmacokinetics

    In brain microvessel endothelial cell monolayers, DSIP flux was insensitive to metabolic inhibitors.

    In plain wordsIn lab-grown brain barrier cells, blocking cell metabolism did not change DSIP crossing.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    No significant changeEffect of metabolic inhibitors on DSIP flux
    Study

    n: not reported in source

    Primary cultures of brain microvessel endothelial cell (BMEC) monolayers in side-by-side diffusion apparatus
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    insensitive to metabolic inhibitors, and not altered by high concentrations of tryptophan.
    1 context sentence
    Summary · context
    The diffusion of delta sleep-inducing peptide (DSIP) across the blood-brain barrier (BBB) has been investigated with an in-vitro model comprised of primary cultures of brain microvessel endothelial cell (BMEC) monolayers.
  6. 06LabIn vitro (cells)Pharmacokinetics

    In brain microvessel endothelial cell monolayers, high concentrations of tryptophan did not alter DSIP flux.

    In plain wordsIn lab-grown brain barrier cells, high tryptophan levels did not change DSIP crossing.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    No significant changeEffect of tryptophan on DSIP flux
    Study

    n: not reported in source

    Primary cultures of brain microvessel endothelial cell (BMEC) monolayers in side-by-side diffusion apparatus
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    not altered by high concentrations of tryptophan.
    1 context sentence
    Summary · context
    The diffusion of delta sleep-inducing peptide (DSIP) across the blood-brain barrier (BBB) has been investigated with an in-vitro model comprised of primary cultures of brain microvessel endothelial cell (BMEC) monolayers.
  7. 07LabIn vitro (cells)Pharmacokinetics

    In brain microvessel endothelial cell monolayers, little degradation of DSIP to tryptophan and des-trp DSIP occurred, with an apparent half-life of about 10 h.

    In plain wordsIn lab-grown brain barrier cells, DSIP broke down only slowly during the experiments.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableDSIP degradation during diffusion experiments
    Study

    n: not reported in source

    Primary cultures of brain microvessel endothelial cell (BMEC) monolayers in side-by-side diffusion apparatus
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Apparent t1/2
    ≈ 10 h
    DSIP degradation

    Source sentences

    Summary
    Little degradation (apparent t1/2 about 10 h) of DSIP to major metabolites, tryptophan (trp) and des-trp DSIP, occurred over the time of the diffusion experiments.
    1 context sentence
    Summary · context
    The diffusion of delta sleep-inducing peptide (DSIP) across the blood-brain barrier (BBB) has been investigated with an in-vitro model comprised of primary cultures of brain microvessel endothelial cell (BMEC) monolayers.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

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