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Study · Animal study (in vivo) · guinea pig · 1989

5 results

Saturable mechanism for delta sleep-inducing peptide (DSIP) at the blood-brain barrier of the vascularly perfused guinea pig brain.

Zlokovic BV, Susic VT, Davson H, Begley DJ, Jankov RM, Mitrovic DM, Lipovac MN

Peptides1 Mar 1989Cited by 18 (Europe PMC)

PMID2547200 (opens in a new tab)DOI10.1016/0196-9781(89)90026-0 (opens in a new tab)PepBankPUB-02547200
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Pharmacokinetics

    In perfused guinea pig forebrain, regional Kin values for [125I]-DSIP were highest in hippocampus, then caudate nucleus, then parietal cortex.

    In plain wordsIn guinea pigs, labelled DSIP entered the hippocampus fastest, then the caudate nucleus, then the parietal cortex.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableRegional unidirectional transfer constant (Kin) of [125I]-DSIP
    Study

    guinea pign: not reported in source

    Ipsilateral perfused in situ forebrain
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated · in situ vascular perfusionAs given in this study (guinea pig).

    Measurements

    Kin
    0.93 microliter.min-1 g-1
    parietal cortex
    Kin
    1.33 microliter.min-1 g-1
    caudate nucleus
    Kin
    1.66 microliter.min-1 g-1
    hippocampus

    Source sentences

    Summary
    Regional unidirectional transfer constants (Kin) calculated from the multiple-time brain uptake analysis were 0.93, 1.33 and 1.66 microliter.min-1 g-1 for the parietal cortex, caudate nucleus and hippocampus, respectively.
    1 context sentence
    Summary · context
    Cellular uptake of [125I] labelled DSIP at the luminal interface of the blood-brain barrier (BBB) was studied in the ipsilateral perfused in situ guinea pig forebrain.
  2. 02AnimalAnimal study (in vivo)Pharmacokinetics

    In perfused guinea pig forebrain, unlabelled DSIP inhibited brain uptake of [125I]-DSIP, reducing Kin to values comparable with mannitol.

    In plain wordsIn guinea pigs, extra unlabelled DSIP reduced the brain uptake of labelled DSIP to the level of mannitol.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    DecreaseInhibition of [125I]-DSIP brain uptake by unlabelled DSIP
    Study

    guinea pign: not reported in source

    Ipsilateral perfused in situ forebrain
    Comparator
    Without unlabelled DSIP
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    0.3 nM [125I]-DSIP with 7 microM unlabelled DSIP · in situ vascular perfusionAs given in this study (guinea pig).

    Measurements

    Kin
    0.23 to 0.38 microliter.min-1 g-1
    with 7 microM unlabelled DSIP

    Source sentences

    Summary
    In the presence of 7 microM unlabelled DSIP the brain uptake of [125I]-DSIP (0.3 nM) was inhibited, the values of Kin being reduced to 0.23-0.38 microliter.min-1 g-1, values that were comparable with the Kin for mannitol.
    1 context sentence
    Summary · context
    Cellular uptake of [125I] labelled DSIP at the luminal interface of the blood-brain barrier (BBB) was studied in the ipsilateral perfused in situ guinea pig forebrain.
  3. 03AnimalAnimal study (in vivo)Pharmacokinetics

    In perfused guinea pig forebrain, the rapidly equilibrating space Vi was greater for [125I]-DSIP than for mannitol, and unlabelled DSIP reduced it to that of mannitol.

    In plain wordsIn guinea pigs, labelled DSIP filled a larger brain space than mannitol, which suggests binding at specific sites on brain capillaries.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    IncreaseRapidly equilibrating space (Vi) of [125I]-DSIP
    Study

    guinea pign: not reported in source

    Ipsilateral perfused in situ forebrain
    Comparator
    Mannitol
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated · in situ vascular perfusionAs given in this study (guinea pig).

    Source sentences

    Summary
    The rapidly equilibrating space of brain, measured from the intercept of the line describing brain uptake versus time on the brain uptake ordinate, Vi, was greater for [125I]-DSIP than for mannitol; in the presence of unlabelled DSIP this was reduced to that of mannitol
    1 context sentence
    Summary · context
    Cellular uptake of [125I] labelled DSIP at the luminal interface of the blood-brain barrier (BBB) was studied in the ipsilateral perfused in situ guinea pig forebrain.
  4. 04AnimalAnimal study (in vivo)Pharmacokinetics

    In perfused guinea pig forebrain, L-tryptophan inhibited the transfer constant Kin of [125I]-DSIP without affecting Vi.

    In plain wordsIn guinea pigs, the amino acid tryptophan reduced the uptake rate of labelled DSIP into the brain.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    DecreaseEffect of L-tryptophan on DSIP Kin and Vi
    Study

    guinea pign: not reported in source

    Ipsilateral perfused in situ forebrain
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    L-tryptophan 7 microM and 1 mM · in situ vascular perfusionAs given in this study (guinea pig).

    Source sentences

    Summary
    L-tryptophan, the N-terminal residue of DSIP, in concentrations of 7 microM and 1 mM, inhibited Kin without affecting Vi.
    1 context sentence
    Summary · context
    Cellular uptake of [125I] labelled DSIP at the luminal interface of the blood-brain barrier (BBB) was studied in the ipsilateral perfused in situ guinea pig forebrain.
  5. 05AnimalAnimal study (in vivo)Pharmacokinetics

    In perfused guinea pig forebrain, vasopressin ([Arg8]-VP) moderately inhibited Kin of [125I]-DSIP, but only at a high concentration.

    In plain wordsIn guinea pigs, vasopressin only moderately reduced DSIP uptake into the brain, and only at a very high concentration.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    DecreaseEffect of vasopressin on DSIP Kin
    Study

    guinea pign: not reported in source

    Ipsilateral perfused in situ forebrain
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    [Arg8]-VP 0.2 mM · in situ vascular perfusionAs given in this study (guinea pig).

    Source sentences

    Summary
    A moderate inhibition of Kin was obtained by vasopressin ([Arg8]-VP), but only at a concentration as high as 0.2 mM.
    1 context sentence
    Summary · context
    Cellular uptake of [125I] labelled DSIP at the luminal interface of the blood-brain barrier (BBB) was studied in the ipsilateral perfused in situ guinea pig forebrain.

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

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