The synergistic effects of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 on growth hormone (GH)-releasing factor-stimulated GH release and intracellular adenosine 3',5'-monophosphate accumulation in rat primary pituitary cell culture.

In rat primary pituitary cells, GRF increased intracellular cAMP concentrations 3-fold.

In plain wordsIn cells, GH-releasing factor raised cAMP, an internal signal molecule, 3-fold.

Measurements

Intracellular cAMP increase

3-fold

GRF

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In rat primary pituitary cells, GRF and GHRP-6 together at maximal concentrations produced a synergistic effect on GH release.

In plain wordsIn cells, GH-releasing factor together with GHRP-6 released more growth hormone than expected from each alone.

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In rat primary pituitary cells, combined GRF and GHRP-6 potentiated the GRF-induced increase in cAMP levels.

In plain wordsIn cells, adding GHRP-6 to GH-releasing factor increased the cAMP rise more than GH-releasing factor alone.

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In rat primary pituitary cells, pretreatment with GRF for 1 h had no effect on the later GH release by GRF or GHRP-6.

In plain wordsIn cells, one hour of earlier GH-releasing factor exposure did not change later growth hormone release by it or by GHRP-6.

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In rat primary pituitary cells, GHRP‑6 stimulated GH release in a time- and dose-dependent manner.

In plain wordsIn rat pituitary cells in lab tests, GHRP‑6 stimulated growth hormone release, with more release at higher doses.

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In rat primary pituitary cells, half-maximal GH stimulation by GHRP‑6 needed 7 x 10(-9) M and maximal stimulation 10(-7) M.

In plain wordsIn rat pituitary cells in lab tests, GHRP‑6 needed very low concentrations to stimulate growth hormone release.

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In rat primary pituitary cells, GHRP‑6 stimulation of GH release was seen after 15 min but not after 4 h of incubation.

In plain wordsIn rat pituitary cells in lab tests, GHRP‑6 stimulated growth hormone release after 15 minutes but not after 4 hours.

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In rat primary pituitary cells, GH release induced by GHRP‑6 was not affected by naloxone or the GRF antagonist.

In plain wordsIn rat pituitary cells in lab tests, GHRP‑6 still released growth hormone when naloxone or a GRF blocker was added.

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In rat primary pituitary cells, GHRP‑6 had no effect on intracellular cAMP levels.

In plain wordsIn rat pituitary cells in lab tests, GHRP‑6 did not change levels of the signal molecule cAMP.

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In rat primary pituitary cells, combined treatment with GRF and GHRP‑6 potentiated the GRF-induced increase in cAMP levels.

In plain wordsIn rat pituitary cells in lab tests, adding GHRP‑6 made the cAMP rise caused by GRF larger.

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In rat primary pituitary cells, 1 h of pretreatment with GHRP‑6 reduced basal GH release by 30%.

In plain wordsIn rat pituitary cells in lab tests, a 1-hour GHRP‑6 pretreatment lowered basal growth hormone release.

Measurements

Reduction in basal GH release

30%

after GHRP-6 pretreatment

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In rat primary pituitary cells, GHRP‑6 pretreatment decreased the subsequent GH response to GHRP‑6.

In plain wordsIn rat pituitary cells in lab tests, earlier GHRP‑6 exposure weakened the later growth hormone response to GHRP‑6.

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In rat primary pituitary cells, GHRP‑6 pretreatment did not decrease the subsequent GH response to GRF.

In plain wordsIn rat pituitary cells in lab tests, earlier GHRP‑6 exposure did not weaken the later response to GRF.

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In rat primary pituitary cells, desensitization induced by GHRP‑6 was completely reversed within 1 h after removal of the peptide.

In plain wordsIn rat pituitary cells in lab tests, the weaker response after GHRP‑6 exposure fully recovered within an hour of removing it.

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