Effects of vasoactive intestinal peptide on steroid secretion and plasminogen activator activity in granulosa cells of the hen.

Johnson AL, Tilly JL

Biology of reproduction1 Mar 1988Cited by 18 (Europe PMC)

PMID2451937DOI10.1095/biolreprod38.2.296PepBankPUB-02451937

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

4 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In isolated hen granulosa cells, VIP induced a dose-related increase in progesterone secretion, with an apparent ED50 of 5.9 X 10(-7) M.
In plain wordsIn lab-grown hen follicle cells, VIP raised progesterone release in a dose-related way.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreaseProgesterone secretion
Study
chickenn: not reported in source
isolated granulosa cells of the largest preovulatory (F1) follicle of the hen
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Vasoactive intestinal peptide, but not avian pancreatic polypeptide, the chicken VIP fragment (16-28) or the VIP congener, PHM-27, induced a dose-related increase in progesterone and androgen secretion, with an apparent median effective dose (ED50) of 5.9 X 10(-7) and 5.7 X 10(-7) M, respectively
1 context sentence(design, population, supporting numbers)
Summary · context
Studies were conducted to evaluate the effects of vasoactive intestinal peptide (VIP) on steroidogenesis and plasminogen-activator (PA) activity in isolated granulosa cells of the largest preovulatory (F1) follicle of the hen.
Report a problemFND-0245193701
02LabIn vitro (cells)Efficacy outcome
In isolated hen granulosa cells, VIP induced a dose-related increase in androgen secretion, with an apparent ED50 of 5.7 X 10(-7) M.
In plain wordsIn lab-grown hen follicle cells, VIP raised androgen release in a dose-related way.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreaseAndrogen secretion
Study
chickenn: not reported in source
isolated granulosa cells of the largest preovulatory (F1) follicle of the hen
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Vasoactive intestinal peptide, but not avian pancreatic polypeptide, the chicken VIP fragment (16-28) or the VIP congener, PHM-27, induced a dose-related increase in progesterone and androgen secretion, with an apparent median effective dose (ED50) of 5.9 X 10(-7) and 5.7 X 10(-7) M, respectively
1 context sentence(design, population, supporting numbers)
Summary · context
Studies were conducted to evaluate the effects of vasoactive intestinal peptide (VIP) on steroidogenesis and plasminogen-activator (PA) activity in isolated granulosa cells of the largest preovulatory (F1) follicle of the hen.
Report a problemFND-0245193702
03LabIn vitro (cells)Mechanism
In isolated hen granulosa cells, VIP acted on steroidogenesis considerably more slowly than luteinizing hormone, attributed to slower induction of cAMP formation.
In plain wordsIn lab-grown hen follicle cells, VIP acted on steroid release more slowly than luteinizing hormone.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableTime course of VIP steroidogenesis versus luteinizing hormone
Study
chickenn: not reported in source
isolated granulosa cells of the largest preovulatory (F1) follicle of the hen
Comparator
Luteinizing hormone
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
However, the time course of action for VIP on steroidogenesis was considerably slower than that for the gonadotropin, luteinizing hormone (LH), and this was attributed to a slower induction of cyclic adenosine 3',5'-monophosphate (cAMP) formation within granulosa cells.
1 context sentence(design, population, supporting numbers)
Summary · context
Studies were conducted to evaluate the effects of vasoactive intestinal peptide (VIP) on steroidogenesis and plasminogen-activator (PA) activity in isolated granulosa cells of the largest preovulatory (F1) follicle of the hen.
Report a problemFND-0245193704
04LabIn vitro (cells)Efficacy outcome
In isolated hen granulosa cells, VIP was a potent inhibitor of plasminogen-activator activity.
In plain wordsIn lab-grown hen follicle cells, VIP strongly reduced plasminogen-activator activity.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
DecreasePlasminogen activator activity
Study
chickenn: not reported in source
isolated granulosa cells of the largest preovulatory (F1) follicle of the hen
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Finally, VIP was found to be a potent inhibitor of PA activity, and this inhibition was potentiated by coincubation of VIP with IBMX.
1 context sentence(design, population, supporting numbers)
Summary · context
Studies were conducted to evaluate the effects of vasoactive intestinal peptide (VIP) on steroidogenesis and plasminogen-activator (PA) activity in isolated granulosa cells of the largest preovulatory (F1) follicle of the hen.
Report a problemFND-0245193705

Studies that did not test the peptide alone2

In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).

05LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cells)Mechanism
In isolated hen granulosa cells, cotreatment with VIP and the phosphodiesterase inhibitor IBMX potentiated the steroidogenic effects of VIP, at least in part through the adenylyl cyclase system.
In plain wordsIn lab-grown hen follicle cells, adding the enzyme blocker IBMX to VIP increased steroid release further.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreaseSteroidogenesis with VIP plus IBMX
Study
chickenn: not reported in source
isolated granulosa cells of the largest preovulatory (F1) follicle of the hen
Comparator
VIP alone
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
The effects of VIP were, at least in part, mediated by the adenylyl cyclase system in that cotreatment of cells with VIP and the phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine (IBMX), potentiated the steroidogenic effects.
1 context sentence(design, population, supporting numbers)
Summary · context
Studies were conducted to evaluate the effects of vasoactive intestinal peptide (VIP) on steroidogenesis and plasminogen-activator (PA) activity in isolated granulosa cells of the largest preovulatory (F1) follicle of the hen.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0245193703
06LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cells)Mechanism
In isolated hen granulosa cells, coincubation of VIP with IBMX potentiated the inhibition of plasminogen-activator activity.
In plain wordsIn lab-grown hen follicle cells, adding IBMX to VIP made the drop in plasminogen-activator activity larger.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
DecreasePlasminogen activator inhibition with VIP plus IBMX
Study
chickenn: not reported in source
isolated granulosa cells of the largest preovulatory (F1) follicle of the hen
Comparator
VIP alone
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
and this inhibition was potentiated by coincubation of VIP with IBMX.
1 context sentence(design, population, supporting numbers)
Summary · context
Studies were conducted to evaluate the effects of vasoactive intestinal peptide (VIP) on steroidogenesis and plasminogen-activator (PA) activity in isolated granulosa cells of the largest preovulatory (F1) follicle of the hen.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0245193706

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
12 Oct 2026, 12:58 UTC
Study results updated
Version 1
9 Oct 2026, 12:41 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

MeSH terms

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