Vasoactive intestinal peptide stimulates androgen biosynthesis by cultured neonatal testicular cells.

Kasson BG, Lim P, Hsueh AJ

Molecular and cellular endocrinology1 Nov 1986Cited by 27 (Europe PMC)

PMID2430845DOI10.1016/0303-7207(86)90162-0PepBankPUB-02430845

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

7 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In cultured neonatal testicular cells, VIP increased testosterone production in a dose-dependent way.
In plain wordsIn cells in a dish, VIP raised testosterone output as the dose went up.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreaseTestosterone production
Study
n: not reported in source
cultured neonatal testicular cells
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
10(-9) to 10(-6) M · 3 daysAs given in this study.
Source sentences
Summary
Treatment of cultured cells with VIP (10(-9) to 10(-6) M) increased the production of testosterone, progesterone, and pregnenolone in a dose-dependent fashion.
1 context sentence(design, population, supporting numbers)
Summary · context
Neonatal testicular cells were treated in vitro with hormones for 3 days and medium steroid or cAMP content was measured by radioimmunoassay.
Report a problemFND-0243084501
02LabIn vitro (cells)Efficacy outcome
In cultured neonatal testicular cells, VIP increased progesterone production in a dose-dependent way.
In plain wordsIn cells in a dish, VIP raised progesterone output as the dose went up.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreaseProgesterone production
Study
n: not reported in source
cultured neonatal testicular cells
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
10(-9) to 10(-6) M · 3 daysAs given in this study.
Source sentences
Summary
Treatment of cultured cells with VIP (10(-9) to 10(-6) M) increased the production of testosterone, progesterone, and pregnenolone in a dose-dependent fashion.
1 context sentence(design, population, supporting numbers)
Summary · context
Neonatal testicular cells were treated in vitro with hormones for 3 days and medium steroid or cAMP content was measured by radioimmunoassay.
Report a problemFND-0243084502
03LabIn vitro (cells)Efficacy outcome
In cultured neonatal testicular cells, VIP increased pregnenolone production in a dose-dependent way.
In plain wordsIn cells in a dish, VIP raised pregnenolone output as the dose went up.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreasePregnenolone production
Study
n: not reported in source
cultured neonatal testicular cells
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
10(-9) to 10(-6) M · 3 daysAs given in this study.
Source sentences
Summary
Treatment of cultured cells with VIP (10(-9) to 10(-6) M) increased the production of testosterone, progesterone, and pregnenolone in a dose-dependent fashion.
1 context sentence(design, population, supporting numbers)
Summary · context
Neonatal testicular cells were treated in vitro with hormones for 3 days and medium steroid or cAMP content was measured by radioimmunoassay.
Report a problemFND-0243084503
04LabIn vitro (cells)Efficacy outcome
In cultured neonatal testicular cells, testosterone production with VIP was a small fraction of the maximal response to LH.
In plain wordsIn cells in a dish, VIP made much less testosterone than the strongest LH response.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableTestosterone production by VIP relative to maximal LH response
Study
n: not reported in source
cultured neonatal testicular cells
Comparator
LH (maximal induction)
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
10(-6) M · 3 daysAs given in this study.
Measurements
Testosterone relative to maximal LH-induced
5 to 10%
10(-6) M VIP
Source sentences
Summary
Testosterone production in response to 10(-6) M VIP was about 5-10% of that maximally induced by LH.
1 context sentence(design, population, supporting numbers)
Summary · context
Neonatal testicular cells were treated in vitro with hormones for 3 days and medium steroid or cAMP content was measured by radioimmunoassay.
Report a problemFND-0243084504
05LabIn vitro (cells)Mechanism
In cultured neonatal testicular cells, VIP stimulated cAMP production in a dose-dependent way.
In plain wordsIn cells in a dish, VIP raised cAMP production as the dose went up.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreasecAMP production
Study
n: not reported in source
cultured neonatal testicular cells
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
Additional experiments also showed a dose-dependent stimulation of cAMP production by VIP.
1 context sentence(design, population, supporting numbers)
Summary · context
Neonatal testicular cells were treated in vitro with hormones for 3 days and medium steroid or cAMP content was measured by radioimmunoassay.
Report a problemFND-0243084508
06LabIn vitro (cells)Mechanism
In an LH-responsive subpopulation of separated testicular cells, VIP stimulated androgen production.
In plain wordsIn cells in a dish, VIP raised androgen output in the group of testis cells that respond to LH.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreaseAndrogen production in LH-responsive testicular cells
Study
n: not reported in source
cultured neonatal testicular cells
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
In these studies, VIP stimulated androgen production in an LH-responsive subpopulation of testis cells
2 context sentences(design, population, supporting numbers)
Summary · context
A direct stimulatory effect of VIP on Leydig cells was indicated in studies on steroidogenesis by testicular cells separated on a metrizamide density gradient.
Summary · context
Neonatal testicular cells were treated in vitro with hormones for 3 days and medium steroid or cAMP content was measured by radioimmunoassay.
Report a problemFND-0243084509
07LabIn vitro (cells)Mechanism
In non-LH-responsive separated testicular cells, VIP did not affect steroid production.
In plain wordsIn cells in a dish, VIP did not change steroid output in the testis cells that do not respond to LH.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
No significant changeSteroid production in non-LH-responsive testicular cells
Study
n: not reported in source
cultured neonatal testicular cells
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
but failed to affect steroid production in non-LH-responsive cells.(ABSTRACT TRUNCATED AT 250 WORDS)
1 context sentence(design, population, supporting numbers)
Summary · context
Neonatal testicular cells were treated in vitro with hormones for 3 days and medium steroid or cAMP content was measured by radioimmunoassay.
Report a problemFND-0243084510

Studies that did not test the peptide alone3

In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).

08LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cells)Mechanism
In cultured neonatal testicular cells, adding the phosphodiesterase inhibitor IBMX to VIP raised testosterone production 13-fold compared with VIP alone.
In plain wordsIn cells in a dish, adding IBMX to VIP raised testosterone output much more than VIP alone.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreaseTestosterone production with VIP plus IBMX
Study
n: not reported in source
cultured neonatal testicular cells
Comparator
VIP alone
Outcome group
Sex hormones and fertility · Endocrine and hormones
Measurements
Testosterone fold increase
13 fold
VIP plus IBMX versus VIP alone
Source sentences
Summary
Addition of methyl-isobutyl-xanthine, a phosphodiesterase inhibitor, to the VIP-containing cultures significantly enhanced production of testosterone by 13-fold, of progesterone by 9-fold, and of pregnenolone by 2.5-fold as compared to treatment with VIP alone.
1 context sentence(design, population, supporting numbers)
Summary · context
Neonatal testicular cells were treated in vitro with hormones for 3 days and medium steroid or cAMP content was measured by radioimmunoassay.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0243084505
09LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cells)Mechanism
In cultured neonatal testicular cells, adding IBMX to VIP raised progesterone production 9-fold compared with VIP alone.
In plain wordsIn cells in a dish, adding IBMX to VIP raised progesterone output much more than VIP alone.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreaseProgesterone production with VIP plus IBMX
Study
n: not reported in source
cultured neonatal testicular cells
Comparator
VIP alone
Outcome group
Sex hormones and fertility · Endocrine and hormones
Measurements
Progesterone fold increase
9 fold
VIP plus IBMX versus VIP alone
Source sentences
Summary
Addition of methyl-isobutyl-xanthine, a phosphodiesterase inhibitor, to the VIP-containing cultures significantly enhanced production of testosterone by 13-fold, of progesterone by 9-fold, and of pregnenolone by 2.5-fold as compared to treatment with VIP alone.
1 context sentence(design, population, supporting numbers)
Summary · context
Neonatal testicular cells were treated in vitro with hormones for 3 days and medium steroid or cAMP content was measured by radioimmunoassay.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0243084506
10LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cells)Mechanism
In cultured neonatal testicular cells, adding IBMX to VIP raised pregnenolone production 2.5-fold compared with VIP alone.
In plain wordsIn cells in a dish, adding IBMX to VIP raised pregnenolone output more than VIP alone.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreasePregnenolone production with VIP plus IBMX
Study
n: not reported in source
cultured neonatal testicular cells
Comparator
VIP alone
Outcome group
Sex hormones and fertility · Endocrine and hormones
Measurements
Pregnenolone fold increase
2.5 fold
VIP plus IBMX versus VIP alone
Source sentences
Summary
Addition of methyl-isobutyl-xanthine, a phosphodiesterase inhibitor, to the VIP-containing cultures significantly enhanced production of testosterone by 13-fold, of progesterone by 9-fold, and of pregnenolone by 2.5-fold as compared to treatment with VIP alone.
1 context sentence(design, population, supporting numbers)
Summary · context
Neonatal testicular cells were treated in vitro with hormones for 3 days and medium steroid or cAMP content was measured by radioimmunoassay.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0243084507

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
12 Oct 2026, 12:58 UTC
Study results updated
Version 1
9 Oct 2026, 12:41 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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