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Study · Measured the body's own peptide · 1990

4 results

Biosynthesis and processing of delta sleep-inducing peptide-like precursors in primary cultures of mouse anterior pituitary cells.

Bjartell A, Ekman R, Loh YP

European journal of biochemistry1 May 1990Cited by 6 (Europe PMC)

PMID2364941 (opens in a new tab)DOI10.1111/j.1432-1033.1990.tb15555.x (opens in a new tab)PepBankPUB-02364941
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Natural levels measured4

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 01LabEndogenous levelIn vitro (cells)Mechanism

    In mouse anterior pituitary cells, three DSIP-immunoprecipitable precursors of 50-60 kDa were processed to intermediates of 35-45 kDa and 9-16.5 kDa.

    In plain wordsIn mouse pituitary cells, the body's own DSIP-like proteins were cut down in steps into smaller pieces.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableDSIP-immunoprecipitable precursors and intermediates
    Study

    mousen: not reported in source

    Primary mouse anterior pituitary cell cultures
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Measurements

    Precursor molecular mass
    50 to 60 kDa
    three precursors
    Intermediate molecular mass
    35 to 45 kDa
    major group
    Intermediate molecular mass
    9 to 16.5 kDa
    major group

    Source sentences

    Summary
    Multiple forms of DSIP-immunoprecipitable material were observed, including three precursors of molecular mass 50-60 kDa which were processed to two major groups of intermediates of 35-45 kDa and 9-16.5 kDa.
    2 context sentences
    Summary · context
    Cells were pulse/chase incubated with 3H-labelled amino acids (Gly, Arg or Ala) and cell extracts were immunoprecipitated with DSIP antiserum.
    Summary · context
    The biosynthesis and processing of material resembling delta sleep-inducing peptide (DSIP) have been studied in mouse anterior pituitary primary cell cultures.

    This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.

  2. 02LabEndogenous levelIn vitro (cells)Mechanism

    In mouse anterior pituitary cells, intermediates appear to be processed to a DSIP-related peptide that co-ran with endogenous mouse DSIP on HPLC but not with rabbit DSIP.

    In plain wordsIn mouse pituitary cells, the body's own small DSIP-like peptide matched mouse DSIP in a lab test but not rabbit DSIP.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableFinal DSIP-related peptide compared with mouse and rabbit DSIP
    Study

    mousen: not reported in source

    Primary mouse anterior pituitary cell cultures
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Measurements

    DSIP-related peptide molecular mass
    < 3 kDa
    mouse anterior pituitary cells

    Source sentences

    Summary
    These intermediates appear to be processed to a DSIP-related peptide (molecular mass less than 3 kDa), which co-ran on reversed-phase HPLC with an endogenous form of DSIP in mouse anterior pituitary, but not with rabbit DSIP.
    1 context sentence
    Summary · context
    The biosynthesis and processing of material resembling delta sleep-inducing peptide (DSIP) have been studied in mouse anterior pituitary primary cell cultures.

    This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.

  3. 03LabEndogenous levelIn vitro (cells)Mechanism

    In mouse anterior pituitary cells, the DSIP-related peptide incorporated [3H]Gly and [3H]glucosamine but not [3H]Arg or [3H]Ala, indicating a glycopeptide.

    In plain wordsIn mouse pituitary cells, the body's own small DSIP-like peptide carried a sugar group, so it is a glycopeptide.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableGlycopeptide nature of DSIP-related peptide
    Study

    mousen: not reported in source

    Primary mouse anterior pituitary cell cultures
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    This less than 3-kDa peptide incorporated [3H]Gly, but not [3H]Arg or [3H]Ala. In addition, it incorporated [3H]glucosamine, indicating that it was a glycopeptide.
    1 context sentence
    Summary · context
    The biosynthesis and processing of material resembling delta sleep-inducing peptide (DSIP) have been studied in mouse anterior pituitary primary cell cultures.

    This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.

  4. 04LabEndogenous levelIn vitro (cells)Mechanism

    In mouse anterior pituitary cells, the DSIP-like glycopeptide and the 9-16.5-kDa intermediates were released into the medium.

    In plain wordsIn mouse pituitary cells, the body's own small DSIP-like peptide and some larger forms were released into the surrounding fluid.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableSecretion of DSIP-like glycopeptide and intermediates
    Study

    mousen: not reported in source

    Primary mouse anterior pituitary cell cultures
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Measurements

    Intermediate group molecular mass
    9 to 16.5 kDa
    released into medium

    Source sentences

    Summary
    Secretion studies showed release of the less than 3-kDa DSIP-like glycopeptide and the 9-16.5-kDa group of intermediates into the medium.
    1 context sentence
    Summary · context
    The biosynthesis and processing of material resembling delta sleep-inducing peptide (DSIP) have been studied in mouse anterior pituitary primary cell cultures.

    This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    9 Oct 2026, 18:17 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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