Vasoactive intestinal peptide, a singlet oxygen quencher.

Misra BR, Misra HP

The Journal of biological chemistry1 Sept 1990Cited by 56 (Europe PMC)

PMID2168410DOI10.1016/s0021-9258(18)55404-1PepBankPUB-02168410

Publisher page PubMedAsk about this study

Summary

The neuropeptide vasoactive intestinal peptide (VIP), a highly basic 28-amino acid peptide, has a widespread distribution in the body. The functional specificity of this peptide not only includes its potent vasodilatory activity, but also its role in protecting lungs against acute injury, in preventing T-lymphocyte proliferation and in modulating immune function. We have investigated the possible antioxidant properties of VIP and found that VIP does not have significant O2-, OH., or H2O2 scavenging ability. However, VIP was found to inhibit, in a dose-dependent manner, the 1O2-dependent 2,2,6,6-tetramethylpiperidine N-oxyl (TEMPO) formation. 1O2 was produced in photosensitizing systems using rose bengal or methylene blue as sensitizers and was detected as TEMP-1O2 product (TEMPO) by electron paramagnetic resonance (EPR) spectroscopic techniques. The formation of TEMPO signal was strongly inhibited by known singlet quenchers, e.g. beta-carotene, histidine as well as azide, but not by catalase (20 micrograms/ml) which removes H2O2 and mannitol (6 mM) or ethanol (5.9 mM) which remove OH.. Superoxide dismutase (2.5 micrograms/ml) inhibited the photoreaction up to 20% by removing O2- and most probably by blocking the secondary charge transfer pathway of 1O2 formation. These results suggest that the formation of nitroxide radical by 1O2 attack on TEMP may be used as a simple and specific assay for 1O2, and VIP can serve as an effective 1O2 scavenger/quencher, thus it may modulate the oxidative tissue injury caused by this reactive species of oxygen.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cell-free)Activity measurement
In a cell-free assay, VIP did not show significant superoxide scavenging ability.
In plain wordsIn lab tests, VIP did not clear superoxide radicals to a significant degree.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
No significant changeSuperoxide (O2-) scavenging
Study
n: not reported in source
Outcome group
Oxidative stress · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
We have investigated the possible antioxidant properties of VIP and found that VIP does not have significant O2-, OH., or H2O2 scavenging ability.
1 context sentence(design, population, supporting numbers)
Summary · context
1O2 was produced in photosensitizing systems using rose bengal or methylene blue as sensitizers and was detected as TEMP-1O2 product (TEMPO) by electron paramagnetic resonance (EPR) spectroscopic techniques.
Show in the study text Report a problemFND-0216841001
02LabIn vitro (cell-free)Activity measurement
In a cell-free assay, VIP did not show significant hydroxyl radical scavenging ability.
In plain wordsIn lab tests, VIP did not clear hydroxyl radicals to a significant degree.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
No significant changeHydroxyl radical (OH.) scavenging
Study
n: not reported in source
Outcome group
Oxidative stress · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
We have investigated the possible antioxidant properties of VIP and found that VIP does not have significant O2-, OH., or H2O2 scavenging ability.
1 context sentence(design, population, supporting numbers)
Summary · context
1O2 was produced in photosensitizing systems using rose bengal or methylene blue as sensitizers and was detected as TEMP-1O2 product (TEMPO) by electron paramagnetic resonance (EPR) spectroscopic techniques.
Show in the study text Report a problemFND-0216841002
03LabIn vitro (cell-free)Activity measurement
In a cell-free assay, VIP did not show significant hydrogen peroxide scavenging ability.
In plain wordsIn lab tests, VIP did not clear hydrogen peroxide to a significant degree.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
No significant changeHydrogen peroxide (H2O2) scavenging
Study
n: not reported in source
Outcome group
Oxidative stress · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
We have investigated the possible antioxidant properties of VIP and found that VIP does not have significant O2-, OH., or H2O2 scavenging ability.
1 context sentence(design, population, supporting numbers)
Summary · context
1O2 was produced in photosensitizing systems using rose bengal or methylene blue as sensitizers and was detected as TEMP-1O2 product (TEMPO) by electron paramagnetic resonance (EPR) spectroscopic techniques.
Show in the study text Report a problemFND-0216841003
04LabIn vitro (cell-free)Activity measurement
In a cell-free photosensitizing system, VIP inhibited singlet oxygen-dependent TEMPO formation in a dose-dependent manner.
In plain wordsIn lab tests, VIP reduced singlet oxygen activity in a dose-dependent way.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
DecreaseSinglet oxygen (1O2) quenching, TEMPO formation
Study
n: not reported in source
Outcome group
Oxidative stress · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
However, VIP was found to inhibit, in a dose-dependent manner, the 1O2-dependent 2,2,6,6-tetramethylpiperidine N-oxyl (TEMPO) formation.
1 context sentence(design, population, supporting numbers)
Summary · context
1O2 was produced in photosensitizing systems using rose bengal or methylene blue as sensitizers and was detected as TEMP-1O2 product (TEMPO) by electron paramagnetic resonance (EPR) spectroscopic techniques.
Show in the study text Report a problemFND-0216841004

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
12 Oct 2026, 12:58 UTC
Study results updated
Version 1
9 Oct 2026, 12:41 UTC
First added · source update from Europe PMC

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