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Study · Not a test of the peptide alone · 1990

3 results

Oxytocin receptors from LLC-PK1 cells: expression in Xenopus oocytes.

Cantau B, Barjon JN, Chicot D, Baskevitch PP, Jard S

The American journal of physiology1 Apr 1990Cited by 7 (Europe PMC)

PMID2158746 (opens in a new tab)DOI10.1152/ajprenal.1990.258.4.f963 (opens in a new tab)PepBankPUB-02158746
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone3

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

  1. 01LabNot a test of Oxytocin aloneIn vitro (cells)Activity measurement

    Radioligand analogs of oxytocin bound with high affinity to the same population of sites on LLC-PK1 cell membranes, with mean Kd values of 14 and 0.06 nM.

    In plain wordsIn pig kidney cell membranes, two oxytocin-like labels bound tightly to the same oxytocin receptor sites.

    Peptide
    Oxytocin
    Outcome
    Not applicableBinding affinity of oxytocin receptor ligands to LLC-PK1 membranes
    Study

    pign: not reported in source

    LLC-PK1 and LLC-PK1L pig kidney cell lines
    Outcome group
    Receptor activity and mechanism · Kidney and urinary

    Measurements

    Kd
    14 nM
    [3H]-[Thr4,Gly7]OT
    Kd
    0.06 nM
    125I-OTA
    Bmax
    100 fmol/mg membrane protein
    LLC-PK1 membranes

    Source sentences

    Summary
    [3H]-[Thr4,Gly7]OT and 125I-OTA bind with high affinity (mean Kd values of 14 and 0.06 nM, respectively) to the same population of sites on LLC-PK1 cell membranes [maximum binding (Bmax) of 100 fmol/mg membrane protein].
    1 context sentence
    Summary · context
    Two selective radioligands for oxytocin receptors, [3H]-[4-threonine,7-glycine]oxytocin [( 3H]-[Thr4,Gly7]OT) and 125I-[1-(beta-mercapto-beta,beta-cyclopentamethylenepropionic acid), 2-(O-methyl)tyrosine, 4-threonine, 8-ornithine, 9-tyrosine amide]-oxytocin (125I-OTA), were used to characterize oxyt…

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  2. 02LabNot a test of Oxytocin aloneIn vitro (cells)Activity measurement

    In LLC-PK1 cells, the oxytocin analog [Thr4,Gly7]OT affected neither basal cAMP content nor vasopressin-induced cAMP accumulation.

    In plain wordsIn pig kidney cells, an oxytocin-like peptide did not change cAMP, so these receptors are not linked to adenylate cyclase.

    Peptide
    Oxytocin
    Outcome
    No significant changeAdenylate cyclase coupling of oxytocin receptors
    Study

    pign: not reported in source

    LLC-PK1 and LLC-PK1L pig kidney cell lines
    Outcome group
    Receptor activity and mechanism · Kidney and urinary

    Source sentences

    Summary
    Oxytocin receptors from LLC-PK1 cells have no functional relationship with adenylate cyclase. [Thr4,Gly7]OT affected neither the basal adenosine 3',5'-cyclic monophosphate (cAMP) content nor the vasopressin-induced cAMP accumulation by LLC-PK1 cells.
    1 context sentence
    Summary · context
    Two selective radioligands for oxytocin receptors, [3H]-[4-threonine,7-glycine]oxytocin [( 3H]-[Thr4,Gly7]OT) and 125I-[1-(beta-mercapto-beta,beta-cyclopentamethylenepropionic acid), 2-(O-methyl)tyrosine, 4-threonine, 8-ornithine, 9-tyrosine amide]-oxytocin (125I-OTA), were used to characterize oxyt…

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  3. 03LabNot a test of Oxytocin aloneIn vitro (cells)Activity measurement

    In Xenopus oocytes injected with LLC-PK1 cell mRNA, [Thr4,Gly7]OT increased 45Ca2+ outflux, and a selective oxytocin antagonist blocked this effect.

    In plain wordsIn frog egg cells carrying pig kidney receptor mRNA, an oxytocin-like peptide raised calcium outflow, and an oxytocin blocker stopped it.

    Peptide
    Oxytocin
    Outcome
    Increase45Ca2+ outflux in Xenopus oocytes injected with LLC-PK1 mRNA
    Study

    pign: not reported in source

    LLC-PK1 and LLC-PK1L pig kidney cell lines
    Outcome group
    Receptor activity and mechanism · Kidney and urinary

    Source sentences

    Summary
    Xenopus laevis oocytes injected with LLC-PK1 cell mRNA responded to [Thr4,Gly7]OT by an increase in 45Ca2+ outflux; this effect is antagonized by a highly selective oxytocin antagonist.
    1 context sentence
    Summary · context
    Two selective radioligands for oxytocin receptors, [3H]-[4-threonine,7-glycine]oxytocin [( 3H]-[Thr4,Gly7]OT) and 125I-[1-(beta-mercapto-beta,beta-cyclopentamethylenepropionic acid), 2-(O-methyl)tyrosine, 4-threonine, 8-ornithine, 9-tyrosine amide]-oxytocin (125I-OTA), were used to characterize oxyt…

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    11 Oct 2026, 20:03 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:52 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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