In rat lung preparations, VIP receptors extracted with digitonin kept the ability to bind 125I-VIP, while those solubilized in Triton X-100 showed little or no activity.
In plain wordsIn lab tests, rat lung VIP receptors kept binding VIP after extraction with digitonin but not after extraction with Triton X-100.
- Outcome
- Not applicable125I-VIP binding of detergent-solubilized receptors
- Study
ratn: not reported in source
rat lung VIP receptor, membrane-bound and detergent-solubilized- Outcome group
- Receptor activity and mechanism · Pharmacology
Source sentences
Receptors that were extracted from the membrane using digitonin retained the ability to bind 125I-VIP, while those solubilized in Triton X-100 displayed little or no detectable activity.
1 context sentence(design, population, supporting numbers)
Limited proteolysis was used to probe and compare the conformation of the rat lung vasoactive intestinal peptide (VIP) receptor in membrane-bound and detergent-solubilized states.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).