Colocalization of delta sleep inducing peptide and luteinizing hormone releasing hormone in neurosecretory vesicles in rat median eminence.

Vallet PG, Charnay Y, Boura C, Kiss JZ

Neuroendocrinology1 Jan 1991Cited by 13 (Europe PMC)

PMID2046856DOI10.1159/000125705PepBankPUB-02046856

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

No result from this study comes from a test of the peptide.

Natural levels measured6

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01AnimalEndogenous levelAnimal study (in vivo)Other
In the rat median eminence, DSIP- and LH-RH-immunostained fibers and varicosities showed a similar distribution pattern.
In plain wordsIn rats, natural DSIP-like material and LH-RH were found in similar places in the median eminence.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicableDistribution of DSIP and LH-RH immunostaining in median eminence
Study
ratn: not reported in source
median eminence sections
Outcome group
Endogenous peptide levels · Nervous system and cognition
Source sentences
Summary
At the light microscopic level, DSIP and LH-RH immunostained fibers, and varicosities exhibited a similar distribution pattern throughout the median eminence.
1 context sentence(design, population, supporting numbers)
Summary · context
The colocalization of immunoreactivities similar to delta sleep inducing peptide (DSIP) and luteinizing hormone releasing hormone (LH-RH) was investigated by light and electron microscopic immunocytochemistry in the rat median eminence.
This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0204685601
02AnimalEndogenous levelAnimal study (in vivo)Mechanism
In the rat median eminence, an elution-restaining technique showed coexistence of LH-RH and DSIP immunoreactivities in most labelled axons.
In plain wordsIn rats, natural DSIP-like material and LH-RH were found together in most labelled nerve fibres.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicableCoexistence of LH-RH and DSIP immunoreactivities in axons
Study
ratn: not reported in source
median eminence sections
Outcome group
Endogenous peptide levels · Nervous system and cognition
Source sentences
Summary
Using an elution-restaining technique, the coexistence of LH-RH and DSIP immunoreactivities was observed in most labelled axons.
1 context sentence(design, population, supporting numbers)
Summary · context
The colocalization of immunoreactivities similar to delta sleep inducing peptide (DSIP) and luteinizing hormone releasing hormone (LH-RH) was investigated by light and electron microscopic immunocytochemistry in the rat median eminence.
This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0204685602
03AnimalEndogenous levelAnimal study (in vivo)Mechanism
In the rat median eminence, double immunogold labelling found DSIP and LH-RH colocalized in single axons.
In plain wordsIn rats, natural DSIP-like material and LH-RH were found in the same single nerve fibres.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicableColocalization of DSIP and LH-RH in single axons by electron microscopy
Study
ratn: not reported in source
median eminence sections
Outcome group
Endogenous peptide levels · Nervous system and cognition
Source sentences
Summary
The two peptides were found colocalized in single axons.
2 context sentences(design, population, supporting numbers)
Summary · context
To determine the intracellular localization of DSIP and LH-RH, we used double immunocytochemical labelling with species-specific antibodies and secondary antibodies conjugated to different sizes of gold particles.
Summary · context
The colocalization of immunoreactivities similar to delta sleep inducing peptide (DSIP) and luteinizing hormone releasing hormone (LH-RH) was investigated by light and electron microscopic immunocytochemistry in the rat median eminence.
This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0204685603
04AnimalEndogenous levelAnimal study (in vivo)Mechanism
In the rat median eminence, LH-RH and DSIP (or a closely related form) were contained in the same dense-core vesicles of about 100 nm, which suggests cosecretion.
In plain wordsIn rats, natural DSIP-like material and LH-RH were stored in the same small vesicles, which suggests they are released together.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicableColocalization of DSIP and LH-RH in dense-core vesicles
Study
ratn: not reported in source
median eminence sections
Outcome group
Endogenous peptide levels · Nervous system and cognition
Source sentences
Summary
Moreover, we could demonstrate that LH-RH and DSIP (or a closely related molecular form) are contained not only in the same axons, but also in the same approximately 100-nm dense-core vesicles, suggesting cosecretion of these peptides.
1 context sentence(design, population, supporting numbers)
Summary · context
The colocalization of immunoreactivities similar to delta sleep inducing peptide (DSIP) and luteinizing hormone releasing hormone (LH-RH) was investigated by light and electron microscopic immunocytochemistry in the rat median eminence.
This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0204685604
05AnimalEndogenous levelAnimal study (in vivo)Other
In the rat median eminence, no DSIP staining was observed in tanycytes or ependymal or glial elements.
In plain wordsIn rats, no natural DSIP-like material was seen in support cells of the median eminence.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicableDSIP staining in tanycytes, ependymal and glial elements
Study
ratn: not reported in source
median eminence sections
Outcome group
Endogenous peptide levels · Nervous system and cognition
Source sentences
Summary
No staining was observed in tanycytes and ependymal or glial elements.
1 context sentence(design, population, supporting numbers)
Summary · context
The colocalization of immunoreactivities similar to delta sleep inducing peptide (DSIP) and luteinizing hormone releasing hormone (LH-RH) was investigated by light and electron microscopic immunocytochemistry in the rat median eminence.
This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0204685605
06AnimalEndogenous levelAnimal study (in vivo)Other
In the rat median eminence, immunoreactive terminals frequently showed direct membrane apposition with tanycyte processes but rare contacts with portal capillaries.
In plain wordsIn rats, nerve endings with natural DSIP-like material often touched support cells called tanycytes and rarely touched blood capillaries.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicableContacts of DSIP- and LH-RH-immunoreactive terminals with tanycytes and capillaries
Study
ratn: not reported in source
median eminence sections
Outcome group
Endogenous peptide levels · Nervous system and cognition
Source sentences
Summary
Immunoreactive terminals frequently showed direct membrane apposition with tanycyte processes but rare contacts with portal capillaries.
This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0204685606

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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