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Study · In vitro (cells) · pig · 1990

4 results

N omega-nitro-L-arginine: a potent inhibitor of the L-arginine-dependent soluble guanylate cyclase activation pathway in LLC-PK1 cells.

Ishii K, Kerwin JF Jr, Murad F

Canadian journal of physiology and pharmacology1 Jun 1990Cited by 17 (Europe PMC)

PMID1973629 (opens in a new tab)DOI10.1139/y90-114 (opens in a new tab)PepBankPUB-01973629
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

1 result from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In LLC-PK1 porcine kidney epithelial cells, oxytocin increased cyclic GMP levels through activation of soluble guanylate cyclase.

    In plain wordsIn pig kidney cells, oxytocin raised cyclic GMP, a cell signal, by switching on an enzyme.

    Peptide
    Oxytocin
    Outcome
    IncreaseCyclic GMP level after oxytocin
    Study

    pign: not reported in source

    LLC-PK1 porcine kidney epithelial cells
    Outcome group
    Receptor activity and mechanism · Kidney and urinary
    Studied dose
    10 microMAs given in this study (pig).

    Source sentences

    Summary
    Oxytocin increased cyclic GMP levels in LLC-PK1 porcine kidney epithelial cells through activation of soluble guanylate cyclase.
    1 context sentence
    Summary · context
    oxytocin (10 microM) induced cyclic GMP accumulation

Studies that did not test the peptide alone3

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

  1. 02LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism

    In LLC-PK1 cells, NG-monomethyl-L-arginine inhibited oxytocin-induced cyclic GMP accumulation with an IC50 of 2.3 microM.

    In plain wordsIn pig kidney cells, a nitric-oxide-pathway blocker reduced the cyclic GMP rise caused by oxytocin.

    Peptide
    Oxytocin
    Outcome
    DecreaseInhibition of oxytocin-induced cyclic GMP by NG-monomethyl-L-arginine
    Study

    pign: not reported in source

    LLC-PK1 porcine kidney epithelial cells
    Outcome group
    Receptor activity and mechanism · Kidney and urinary

    Measurements

    IC50
    2.3 microM
    NG-monomethyl-L-arginine

    Source sentences

    Summary
    NG-Monomethyl-L-arginine and N omega-nitro-L-arginine inhibited oxytocin (10 microM) induced cyclic GMP accumulation with IC50 values of 2.3 microM and 140 nM, respectively

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  2. 03LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism

    In LLC-PK1 cells, N omega-nitro-L-arginine inhibited oxytocin-induced cyclic GMP accumulation with an IC50 of 140 nM.

    In plain wordsIn pig kidney cells, a second nitric-oxide-pathway blocker reduced the cyclic GMP rise caused by oxytocin, at a lower concentration.

    Peptide
    Oxytocin
    Outcome
    DecreaseInhibition of oxytocin-induced cyclic GMP by N omega-nitro-L-arginine
    Study

    pign: not reported in source

    LLC-PK1 porcine kidney epithelial cells
    Outcome group
    Receptor activity and mechanism · Kidney and urinary

    Measurements

    IC50
    140 nM
    N omega-nitro-L-arginine

    Source sentences

    Summary
    NG-Monomethyl-L-arginine and N omega-nitro-L-arginine inhibited oxytocin (10 microM) induced cyclic GMP accumulation with IC50 values of 2.3 microM and 140 nM, respectively

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  3. 04LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism

    In LLC-PK1 cells, the inhibition of oxytocin-induced cyclic GMP accumulation was prevented by L-arginine.

    In plain wordsIn pig kidney cells, adding L-arginine stopped the blockers from reducing the oxytocin-induced cyclic GMP rise.

    Peptide
    Oxytocin
    Outcome
    Not applicableReversal of inhibition by L-arginine
    Study

    pign: not reported in source

    LLC-PK1 porcine kidney epithelial cells
    Outcome group
    Receptor activity and mechanism · Kidney and urinary

    Source sentences

    Summary
    and the inhibition was prevented with L-arginine.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    11 Oct 2026, 20:03 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:53 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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