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Study · Ex vivo tissue · rat · 1990

4 results

Vasoactive intestinal peptide receptor on liver plasma membranes: solubilization and cross-linking.

Nguyen TD, Kaiser LM

Peptides1 Nov 1990Cited by 1 (Europe PMC)

PMID1965036 (opens in a new tab)DOI10.1016/0196-9781(90)90160-7 (opens in a new tab)PepBankPUB-01965036
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueActivity measurement

    In rat liver plasma membranes, thymosin alpha‑1 ranked last of the ligands tested for binding to the VIP receptor (VIP greater than PHI greater than secretin greater than thymosin alpha‑1).

    In plain wordsIn lab tests on rat liver membranes, thymosin alpha‑1 bound the VIP receptor less well than VIP, PHI or secretin.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableBinding preference at the hepatic VIP receptor
    Study

    ratn: not reported in source

    rat liver plasma membranes
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    binding preference: VIP greater than PHI greater than secretin greater than thymosin alpha 1
  2. 02LabEx vivo tissueActivity measurement

    In rat liver plasma membranes, the digitonin-solubilized hepatic VIP receptor kept its ability to bind VIP.

    In plain wordsIn lab tests on rat liver membranes, the VIP receptor still bound VIP after it was dissolved out of the membrane.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableVIP receptor binding of the solubilized receptor
    Study

    ratn: not reported in source

    rat liver plasma membranes
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The hepatic receptor for VIP was solubilized from rat liver plasma membranes with 1.4% digitonin and shown to conserve its ability to bind to the ligand.
  3. 03LabEx vivo tissueActivity measurement

    The solubilized rat liver VIP receptor showed high affinity and specificity for VIP, with a KD of about 1 nM.

    In plain wordsIn lab tests on rat liver membranes, the VIP receptor bound VIP tightly and selectively.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableVIP receptor affinity (KD)
    Study

    ratn: not reported in source

    rat liver plasma membranes
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    KD
    ≈ 1 nM
    solubilized VIP receptor, rat liver membranes

    Source sentences

    Summary
    This solubilized receptor demonstrated the high affinity and specificity for VIP (KD approximately 1 nM

Studies that did not test the peptide alone1

In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).

  1. 04LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneEx vivo tissueMechanism

    Cross-linking of 125I-VIP to the solubilized and nonsolubilized rat liver receptors showed a broad band at Mr 56,000 (53,000 without dithiothreitol).

    In plain wordsIn lab tests on rat liver membranes, a labelled VIP cross-linked to a receptor band of about the same size in both preparations.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableApparent size of the cross-linked VIP receptor complex
    Study

    ratn: not reported in source

    rat liver plasma membranes
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Measurements

    apparent Mr
    56,000 Mr
    with dithiothreitol
    apparent Mr
    53,000 Mr
    without dithiothreitol

    Source sentences

    Summary
    A broad autoradiographic band which demonstrated a high affinity for VIP was identified at Mr 56,000 (53,000 in the absence of the reducing agent dithiothreitol) for both the solubilized and nonsolubilized receptors.

    In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 4Current

    10 Oct 2026, 08:55 UTC

    Study results updated

  2. Versions 2 to 3

    9 Oct 2026, 12:41 UTC

    The same notice was added 2 times, first on 9 Oct 2026, 03:03 UTC. The status did not change between them.

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 03:02 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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