Extraction and immunochemical characterization of delta sleep-inducing peptide-like material from the porcine pituitary and adrenal gland.

Bjartell A, Sundler F, Ekman R

Peptides1 May 1991Cited by 3 (Europe PMC)

PMID1923924DOI10.1016/0196-9781(91)90082-zPepBankPUB-01923924

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Natural levels measured4

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01AnimalEndogenous levelAnimal study (in vivo)Other
In porcine anterior pituitary extracts made with water and aprotinin, immunoreactive DSIP reached the highest concentration measured.
In plain wordsIn pig tissue, the highest level of the body's own DSIP-like material was in the anterior pituitary.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicableIR-DSIP concentration in anterior pituitary extracts
Study
pign: not reported in source
Porcine pituitary and adrenal gland extracts
Outcome group
Endogenous peptide levels · Endocrine and hormones
Measurements
IR-DSIP concentration
40.8 ng/g tissue weight
anterior pituitary extract, water with aprotinin
Source sentences
Summary
The highest concentrations were measured in anterior pituitary extracts (40.8 +/- 2.6 ng/g tissue weight) recovered using water with aprotinin.
1 context sentence(design, population, supporting numbers)
Summary · context
In the present study, porcine pituitaries and adrenal glands were extracted in water, saline or acid under various conditions and immunoreactive DSIP (IR-DSIP) quantified by radioimmunoassay.
This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0192392401
02AnimalEndogenous levelAnimal study (in vivo)Other
In porcine adrenal extracts, immunoreactive DSIP had a major peak coeluting with synthetic DSIP(1-9), whereas anterior pituitary extract showed material of higher hydrophobicity.
In plain wordsIn pig adrenal tissue, the body's own DSIP-like material matched synthetic DSIP; pituitary tissue held a more water-repelling form.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicableIR-DSIP elution profile after acetic acid extraction
Study
pign: not reported in source
Porcine pituitary and adrenal gland extracts
Outcome group
Endogenous peptide levels · Endocrine and hormones
Source sentences
Summary
Extraction in acetic acid including C18 Sep-Pak purification resulted in an elution profile of IR-DSIP in adrenal extracts with a major peak coeluting with synthetic DSIP [DSIP(1-9)], whereas anterior pituitary extract showed material of higher hydrophobicity.
1 context sentence(design, population, supporting numbers)
Summary · context
In the present study, porcine pituitaries and adrenal glands were extracted in water, saline or acid under various conditions and immunoreactive DSIP (IR-DSIP) quantified by radioimmunoassay.
This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0192392403
03AnimalEndogenous levelAnimal study (in vivo)Other
In porcine anterior pituitary and adrenal gland extracts, about 30% of immunoreactive DSIP seemed to be glucosylated.
In plain wordsIn pig tissue, about 30% of the body's own DSIP-like material seemed to carry a sugar group.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicableGlucosylation of IR-DSIP
Study
pign: not reported in source
Porcine pituitary and adrenal gland extracts
Outcome group
Endogenous peptide levels · Endocrine and hormones
Measurements
Glucosylated fraction of IR-DSIP
≈ 30%
anterior pituitary and adrenal gland extracts
Source sentences
Summary
Approximately 30% of IR-DSIP in anterior pituitary as well as in adrenal gland extracts seemed to be glucosylated, as based on concanavalin A chromatography.
1 context sentence(design, population, supporting numbers)
Summary · context
In the present study, porcine pituitaries and adrenal glands were extracted in water, saline or acid under various conditions and immunoreactive DSIP (IR-DSIP) quantified by radioimmunoassay.
This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0192392404
04AnimalEndogenous levelAnimal study (in vivo)Other
In porcine pituitary and adrenal gland extracts, immunoblotting identified a 25 kDa DSIP-immunoreactive component.
In plain wordsIn pig tissue, a larger 25 kDa form of the body's own DSIP-like protein was found in both pituitary and adrenal gland.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicable25 kDa DSIP-immunoreactive component
Study
pign: not reported in source
Porcine pituitary and adrenal gland extracts
Outcome group
Endogenous peptide levels · Endocrine and hormones
Measurements
Molecular mass
25 kDa
pituitary and adrenal gland extracts
Source sentences
Summary
One of the DSIP-immunoreactive components by immunoblotting (molecular mass 25 kDa) was identified in both pituitary and adrenal gland extracts.
1 context sentence(design, population, supporting numbers)
Summary · context
In the present study, porcine pituitaries and adrenal glands were extracted in water, saline or acid under various conditions and immunoreactive DSIP (IR-DSIP) quantified by radioimmunoassay.
This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0192392405

Studies that did not test the peptide alone1

In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Delta sleep-inducing peptide (DSIP).

05AnimalNot a test of Delta sleep-inducing peptide (DSIP) aloneAnimal study (in vivo)Other
In porcine tissue extracts, HPLC indicated that hydrophobic forms of immunoreactive DSIP were degraded in water extracts.
In plain wordsIn pig tissue extracts, water extraction degraded some forms of DSIP-like material, so the extraction method matters.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicableDegradation of hydrophobic IR-DSIP forms in water extracts
Study
pign: not reported in source
Porcine pituitary and adrenal gland extracts
Outcome group
Endogenous peptide levels · Endocrine and hormones
Source sentences
Summary
However, high performance liquid chromatography (HPLC) indicated degradation of hydrophobic forms of IR-DSIP in water extracts.
1 context sentence(design, population, supporting numbers)
Summary · context
In the present study, porcine pituitaries and adrenal glands were extracted in water, saline or acid under various conditions and immunoreactive DSIP (IR-DSIP) quantified by radioimmunoassay.
In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Delta sleep-inducing peptide (DSIP).
Report a problemFND-0192392402

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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