Characterization of delta-sleep-inducing peptide-evoked release of Met-enkephalin from brain synaptosomes in rats.

Nakamura A, Sakai K, Takahashi Y, Shiomi H

Journal of neurochemistry1 Sept 1991Cited by 3 (Europe PMC)

PMID1861139DOI10.1111/j.1471-4159.1991.tb08251.xPepBankPUB-01861139

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueActivity measurement
In rat medullary synaptosomes, DSIP significantly increased the release of Met-enkephalin.
In plain wordsIn lab tests on rat brain nerve endings, DSIP increased the release of Met-enkephalin.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
IncreaseMet-enkephalin release from medullary synaptosomes
Study
ratn: not reported in source
Rat brain synaptosomes
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Studied dose
10(-10)-10(-9) MAs given in this study (rat).
Source sentences
Summary
In our present study, DSIP (10(-10)-10(-9) M) induced a significant release of Met-ENK from medullary synaptosomes of rats.
1 context sentence(design, population, supporting numbers)
Summary · context
Delta-sleep-inducing peptide (DSIP) stimulates the release of Met-enkephalin (Met-ENK) from superfused slices of the rodent lower brainstem in vitro.
Report a problemFND-0186113901
02LabEx vivo tissueMechanism
In rat medullary synaptosomes, DSIP-evoked Met-enkephalin release depended on calcium and was not blocked by tetrodotoxin.
In plain wordsIn lab tests on rat brain nerve endings, DSIP-driven Met-enkephalin release needed calcium and was not blocked by tetrodotoxin.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicableCalcium dependence and TTX sensitivity of DSIP-evoked Met-enkephalin release
Study
ratn: not reported in source
Rat brain synaptosomes
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
This DSIP-evoked release of Met-ENK was Ca2+ dependent and tetrodotoxin (TTX) insensitive.
1 context sentence(design, population, supporting numbers)
Summary · context
Delta-sleep-inducing peptide (DSIP) stimulates the release of Met-enkephalin (Met-ENK) from superfused slices of the rodent lower brainstem in vitro.
Report a problemFND-0186113902
03LabEx vivo tissueMechanism
In rat medullary synaptosomes, DSIP significantly increased 45Ca2+ uptake.
In plain wordsIn lab tests on rat brain nerve endings, DSIP increased calcium uptake.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Increase45Ca2+ uptake in medullary synaptosomes
Study
ratn: not reported in source
Rat brain synaptosomes
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Studied dose
10(-11)-10(-9) MAs given in this study (rat).
Source sentences
Summary
Furthermore, DSIP (10(-11)-10(-9) M) significantly increased 45Ca2+ uptake in medullary synaptosomes.
1 context sentence(design, population, supporting numbers)
Summary · context
Delta-sleep-inducing peptide (DSIP) stimulates the release of Met-enkephalin (Met-ENK) from superfused slices of the rodent lower brainstem in vitro.
Report a problemFND-0186113903
04LabEx vivo tissueActivity measurement
In rat synaptosomes, DSIP significantly evoked Met-enkephalin release in the cortex, hypothalamus and midbrain at 10(-10) and 10(-9) M, and in the hippocampus and thalamus only at 10(-9) M.
In plain wordsIn lab tests on rat brain nerve endings, DSIP increased Met-enkephalin release in several brain regions.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
IncreaseMet-enkephalin release in cortex, hypothalamus, midbrain, hippocampus and thalamus
Study
ratn: not reported in source
Rat brain synaptosomes
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Studied dose
10(-10) and 10(-9) MAs given in this study (rat).
Source sentences
Summary
Significant DSIP-evoked Met-ENK release from synaptosomes was observed in the cortex, hypothalamus, and midbrain (at concentrations of 10(-10) and 10(-9) M) and in the hippocampus and thalamus (only at 10(-9) M)
1 context sentence(design, population, supporting numbers)
Summary · context
Delta-sleep-inducing peptide (DSIP) stimulates the release of Met-enkephalin (Met-ENK) from superfused slices of the rodent lower brainstem in vitro.
Report a problemFND-0186113904
05LabEx vivo tissueActivity measurement
In rat striatal synaptosomes, DSIP did not evoke Met-enkephalin release.
In plain wordsIn lab tests on rat brain nerve endings, DSIP did not increase Met-enkephalin release in the striatum.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
No significant changeMet-enkephalin release in striatum
Study
ratn: not reported in source
Rat brain synaptosomes
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
but not in the striatum.
1 context sentence(design, population, supporting numbers)
Summary · context
Delta-sleep-inducing peptide (DSIP) stimulates the release of Met-enkephalin (Met-ENK) from superfused slices of the rodent lower brainstem in vitro.
Report a problemFND-0186113905
06LabEx vivo tissueActivity measurement
In rat hypothalamic synaptosomes, DSIP slightly but not significantly enhanced Leu-enkephalin release.
In plain wordsIn lab tests on rat brain nerve endings, DSIP did not significantly change Leu-enkephalin release in the hypothalamus.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
No significant changeLeu-enkephalin release from hypothalamic synaptosomes
Study
ratn: not reported in source
Rat brain synaptosomes
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Studied dose
10(-10)-10(-8) MAs given in this study (rat).
Source sentences
Summary
In the hypothalamus, the release of Leu-enkephalin from its synaptosomes was slightly, but not significantly, enhanced by DSIP (10(-10)-10(-8) M).
1 context sentence(design, population, supporting numbers)
Summary · context
Delta-sleep-inducing peptide (DSIP) stimulates the release of Met-enkephalin (Met-ENK) from superfused slices of the rodent lower brainstem in vitro.
Report a problemFND-0186113906

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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