False positive result for a peptide drug in the gene conversion assay with Saccharomyces cerevisiae strain D7.

DePass LR, Chan RL, Jagannath DR, Heyman IA

Fundamental and applied toxicology : official journal of the Society of Toxicology1 Oct 1991

PMID1838997DOI10.1016/0272-0590(91)90212-mPepBankPUB-01838997

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

4 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Safety outcome
In lab tests, nafarelin was negative in the Ames assay without S9 activation.
In plain wordsIn lab tests, nafarelin gave a negative Ames result without S9 activation.
Peptide
Nafarelin
Outcome
No significant changeAmes assay without S9
Study
n: not reported in source
Mutagenicity assays
Outcome group
Genotoxicity · Whole body
Studied dose
Dose not stated
Source sentences
Summary
Both tests were negative without S9 activation, and the Ames test was negative with S9, but the yeast test was positive with S9 activation.
2 context sentences(design, population, supporting numbers)
Summary · context
A battery of mutagenicity tests was performed with nafarelin, an agonist analogue of luteinizing hormone releasing hormone (LHRH) containing tryptophan (Trp) and histidine (His).
Summary · context
Included were the Ames assay and the gene conversion assay with yeast strain D7.
Report a problemFND-0183899701
02LabIn vitro (cells)Safety outcome
In lab tests, nafarelin was negative in the yeast gene conversion assay without S9 activation.
In plain wordsIn lab tests, nafarelin gave a negative yeast gene conversion result without S9 activation.
Peptide
Nafarelin
Outcome
No significant changeYeast gene conversion without S9
Study
Saccharomyces cerevisiaen: not reported in source
Yeast strain D7
Outcome group
Genotoxicity · Whole body
Studied dose
Dose not stated
Source sentences
Summary
Both tests were negative without S9 activation, and the Ames test was negative with S9, but the yeast test was positive with S9 activation.
2 context sentences(design, population, supporting numbers)
Summary · context
A battery of mutagenicity tests was performed with nafarelin, an agonist analogue of luteinizing hormone releasing hormone (LHRH) containing tryptophan (Trp) and histidine (His).
Summary · context
Included were the Ames assay and the gene conversion assay with yeast strain D7.
Report a problemFND-0183899702
03LabIn vitro (cells)Safety outcome
In lab tests, nafarelin was negative in the Ames assay with S9 activation.
In plain wordsIn lab tests, nafarelin gave a negative Ames result with S9 activation.
Peptide
Nafarelin
Outcome
No significant changeAmes assay with S9
Study
n: not reported in source
Mutagenicity assays
Outcome group
Genotoxicity · Whole body
Studied dose
Dose not stated
Source sentences
Summary
Both tests were negative without S9 activation, and the Ames test was negative with S9, but the yeast test was positive with S9 activation.
2 context sentences(design, population, supporting numbers)
Summary · context
A battery of mutagenicity tests was performed with nafarelin, an agonist analogue of luteinizing hormone releasing hormone (LHRH) containing tryptophan (Trp) and histidine (His).
Summary · context
Included were the Ames assay and the gene conversion assay with yeast strain D7.
Report a problemFND-0183899703
04LabIn vitro (cells)Safety outcome
In lab tests, nafarelin gave a positive yeast gene conversion result with S9 activation, interpreted as a false positive.
In plain wordsIn lab tests, nafarelin gave a positive yeast result with S9, interpreted as a false positive.
Peptide
Nafarelin
Outcome
IncreaseYeast gene conversion with S9
Study
Saccharomyces cerevisiaen: not reported in source
Yeast strain D7
Outcome group
Genotoxicity · Whole body
Studied dose
Dose not stated
Source sentences
Summary
Both tests were negative without S9 activation, and the Ames test was negative with S9, but the yeast test was positive with S9 activation.
3 context sentences(design, population, supporting numbers)
Summary · context
Since the Ames test is based on mutation to His-independent cells, these data are completely consistent with the negative result in the Ames test and the false positive result in the yeast test.
Summary · context
A battery of mutagenicity tests was performed with nafarelin, an agonist analogue of luteinizing hormone releasing hormone (LHRH) containing tryptophan (Trp) and histidine (His).
Summary · context
Included were the Ames assay and the gene conversion assay with yeast strain D7.
Report a problemFND-0183899704

Studies that did not test the peptide alone2

In this study, Nafarelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Nafarelin.

05LabNot a test of Nafarelin aloneIn vitro (cell-free)Mechanism
In lab tests, amino acid analysis detected tryptophan in the nafarelin incubation mixture.
In plain wordsIn lab tests, tryptophan was detected in the nafarelin incubation mixture.
Peptide
Nafarelin
Outcome
Not applicableTryptophan in incubation mixture
Study
n: not reported in source
Mutagenicity assays
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
In another experiment with the drug, amino acid analysis of the incubation mixture revealed the presence of Trp but no detectable His.
2 context sentences(design, population, supporting numbers)
Summary · context
A battery of mutagenicity tests was performed with nafarelin, an agonist analogue of luteinizing hormone releasing hormone (LHRH) containing tryptophan (Trp) and histidine (His).
Summary · context
Included were the Ames assay and the gene conversion assay with yeast strain D7.
In this study, Nafarelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Nafarelin.
Report a problemFND-0183899705
06LabNot a test of Nafarelin aloneIn vitro (cell-free)Mechanism
In lab tests, amino acid analysis detected no histidine in the nafarelin incubation mixture.
In plain wordsIn lab tests, no histidine was detected in the nafarelin incubation mixture.
Peptide
Nafarelin
Outcome
Not applicableHistidine in incubation mixture
Study
n: not reported in source
Mutagenicity assays
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
In another experiment with the drug, amino acid analysis of the incubation mixture revealed the presence of Trp but no detectable His.
2 context sentences(design, population, supporting numbers)
Summary · context
A battery of mutagenicity tests was performed with nafarelin, an agonist analogue of luteinizing hormone releasing hormone (LHRH) containing tryptophan (Trp) and histidine (His).
Summary · context
Included were the Ames assay and the gene conversion assay with yeast strain D7.
In this study, Nafarelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Nafarelin.
Report a problemFND-0183899706

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
28 Oct 2026, 02:03 UTC
Study results updated
Version 1
27 Oct 2026, 16:36 UTC
First added · source update from Europe PMC

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