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Study · In vitro (cell-free) · 1991

4 results

Phosphorylation of delta sleep-inducing peptide (DSIP) by casein kinase II in vitro.

Nakamura A, Shiomi H

Peptides1 Nov 1991Cited by 1 (Europe PMC)

PMID1815223 (opens in a new tab)DOI10.1016/0196-9781(91)90222-b (opens in a new tab)PepBankPUB-01815223
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cell-free)Activity measurement

    In vitro, casein kinase II phosphorylated DSIP with an apparent Km of 20 mM and Vmax of 90.9 nmol/min/mg protein.

    In plain wordsIn lab tests, the enzyme casein kinase II added a phosphate group to DSIP.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicablePhosphorylation of DSIP by casein kinase II (Km and Vmax)
    Study

    n: not reported in source

    In vitro phosphorylation assay with casein kinase II
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Km
    20 mM
    casein kinase II, DSIP substrate
    Vmax
    90.9 nmol/min/mg protein
    casein kinase II, DSIP substrate

    Source sentences

    Summary
    DSIP was phosphorylated by the enzyme with apparent Km and Vmax values of 20 mM and 90.9 nmol/min/mg protein, respectively.
    1 context sentence
    Summary · context
    In the present study, we examined DSIP as a substrate for in vitro phosphorylation by casein kinase II.
  2. 02LabIn vitro (cell-free)Activity measurement

    In vitro, casein kinase II used both ATP and GTP as phosphoryl donors to phosphorylate DSIP.

    In plain wordsIn lab tests, the enzyme could use either ATP or GTP to add a phosphate group to DSIP.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicablePhosphoryl donor use (ATP and GTP)
    Study

    n: not reported in source

    In vitro phosphorylation assay with casein kinase II
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Both ATP and GTP were utilized as phosphoryl donors.
    1 context sentence
    Summary · context
    In the present study, we examined DSIP as a substrate for in vitro phosphorylation by casein kinase II.
  3. 03LabIn vitro (cell-free)Activity measurement

    In vitro, heparin inhibited the phosphorylation of DSIP by casein kinase II.

    In plain wordsIn lab tests, heparin reduced the addition of a phosphate group to DSIP.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    DecreaseHeparin inhibition of DSIP phosphorylation
    Study

    n: not reported in source

    In vitro phosphorylation assay with casein kinase II
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Phosphorylation of DSIP was inhibited by heparin and enhanced by spermine.
    1 context sentence
    Summary · context
    In the present study, we examined DSIP as a substrate for in vitro phosphorylation by casein kinase II.
  4. 04LabIn vitro (cell-free)Activity measurement

    In vitro, spermine enhanced the phosphorylation of DSIP by casein kinase II.

    In plain wordsIn lab tests, spermine increased the addition of a phosphate group to DSIP.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    IncreaseSpermine enhancement of DSIP phosphorylation
    Study

    n: not reported in source

    In vitro phosphorylation assay with casein kinase II
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    enhanced by spermine.
    1 context sentence
    Summary · context
    In the present study, we examined DSIP as a substrate for in vitro phosphorylation by casein kinase II.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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