Skip to content

Study · Not a test of the peptide alone · 1991

2 results

Orientation of non-blue cupric complexes on DNA fibers.

Chikira M, Sato T, Antholine WE, Petering DH

The Journal of biological chemistry1 Feb 1991Cited by 12 (Europe PMC)

PMID1704368 (opens in a new tab)DOI10.1016/s0021-9258(18)49926-7 (opens in a new tab)PepBankPUB-01704368
Publisher pagePubMedAsk about this study
Jump to 2 results

Summary

Three different orientations of non-blue, type 2 cupric complexes on DNA fibers are obtained from EPR data. The cupric complex of bleomycin, CuBlm, binds as described previously (Shields, H., McGlumphy,C., and Hamrick, P., J., Jr. (1982) Biochim. Biophys. Acta 697, 113-120), except possibly with more restricted motion. The square plane of CuBlm makes an angle of about 65 degrees with the fiber axis. The tridentate complex 2-formylpyridine monothiosemicarbazonato Cu2+ binds with its planar structure perpendicular to the fiber axis. In contrast, other tridentate cupric complexes of tripeptides, CuGHK and CuGHG, bind with the square plane parallel to the fiber axis. The bound forms of Cu(GHK) and Cu(GHG) are determined mostly by the GH moiety in the complex; the contribution of lysine in defining the orientation of the copper moiety is minimal. Thus, the structure of the ligand determines the orientation of these complexes on DNA.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone2

In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHK-Cu.

  1. 01LabNot a test of GHK-Cu aloneIn vitro (cell-free)Mechanism

    In EPR data on DNA fibers, the cupric complexes of the tripeptides GHK and GHG bound with the square plane parallel to the fiber axis.

    In plain wordsIn lab tests, copper-GHK bound to DNA fibers in a set orientation, with its flat plane parallel to the fiber.

    Peptide
    GHK-Cu
    Outcome
    Not applicableOrientation of Cu-GHK on DNA fibers
    Study

    n: not reported in source

    Copper complexes bound to DNA fibers, EPR
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    In contrast, other tridentate cupric complexes of tripeptides, CuGHK and CuGHG, bind with the square plane parallel to the fiber axis.
    1 context sentence
    Summary · context
    Three different orientations of non-blue, type 2 cupric complexes on DNA fibers are obtained from EPR data.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

  2. 02LabNot a test of GHK-Cu aloneIn vitro (cell-free)Mechanism

    In EPR data on DNA fibers, the bound forms of Cu(GHK) and Cu(GHG) were determined mostly by the GH moiety, with minimal contribution of lysine.

    In plain wordsIn lab tests, the GH part of copper-GHK mostly set how it sat on DNA; lysine added little.

    Peptide
    GHK-Cu
    Outcome
    Not applicableDeterminant of Cu(GHK) orientation on DNA fibers
    Study

    n: not reported in source

    Copper complexes bound to DNA fibers, EPR
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    The bound forms of Cu(GHK) and Cu(GHG) are determined mostly by the GH moiety in the complex; the contribution of lysine in defining the orientation of the copper moiety is minimal.
    1 context sentence
    Summary · context
    Three different orientations of non-blue, type 2 cupric complexes on DNA fibers are obtained from EPR data.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    10 Oct 2026, 08:19 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 02:56 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.