Enzymatic degradation of helodermin and vasoactive intestinal polypeptide.

Hachisu M, Hiranuma T, Tani S, Iizuka T

Journal of pharmacobio-dynamics1 Mar 1991Cited by 9 (Europe PMC)

PMID1652635DOI10.1248/bpb1978.14.126PepBankPUB-01652635

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueActivity measurement
In isolated guinea-pig trachea, the relaxing activity of VIP was potentiated by the enkephalinase inhibitors thiorphan and phosphoramidon.
In plain wordsIn lab tests on guinea-pig windpipe, blocking the enzyme enkephalinase made VIP relax the tissue more.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreaseTracheal relaxing activity of VIP with enkephalinase inhibitors
Study
guinea pign: not reported in source
Isolated tracheal tissue
Outcome group
Receptor activity and mechanism · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
The tracheal relaxing activity of VIP was potentiated by enkephalinase inhibitors, thiorphan and phosphoramidon, while the activity of HDM was not potentiated.
1 context sentence(design, population, supporting numbers)
Summary · context
Degradation of HDM in the tracheal tissue isolated from a guinea-pig and by an isolated enkephalinase was studied and compared with the degradation of VIP.
Report a problemFND-0165263501
02LabEx vivo tissueActivity measurement
In isolated guinea-pig trachea, bestatin and captopril did not influence the activity of VIP.
In plain wordsIn lab tests on guinea-pig windpipe, two other enzyme blockers, bestatin and captopril, did not change how VIP worked.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
No significant changeTracheal relaxing activity of VIP with bestatin or captopril
Study
guinea pign: not reported in source
Isolated tracheal tissue
Outcome group
Receptor activity and mechanism · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
On the other hand, bestatin, an aminopeptidase inhibitor, and captopril, an angiotensin converting enzyme inhibitor, did not influence the activity of VIP and HDM.
1 context sentence(design, population, supporting numbers)
Summary · context
Degradation of HDM in the tracheal tissue isolated from a guinea-pig and by an isolated enkephalinase was studied and compared with the degradation of VIP.
Report a problemFND-0165263502
03LabIn vitro (cell-free)Mechanism
Purified enkephalinase from the lung hydrolyzed VIP.
In plain wordsIn lab tests, a purified lung enzyme broke down VIP.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableHydrolysis of VIP by lung enkephalinase
Study
n: not reported in source
Purified enkephalinase from lung
Outcome group
Receptor activity and mechanism · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Enkephalinase was then purified from the lung and the striatum membrane fraction through a DEAE-cellulose column, chromatofocusing column and hydroxyapatite column. The purified enkephalinase from the lung hydrolyzed VIP but not HDM.
1 context sentence(design, population, supporting numbers)
Summary · context
Degradation of HDM in the tracheal tissue isolated from a guinea-pig and by an isolated enkephalinase was studied and compared with the degradation of VIP.
Report a problemFND-0165263503
04LabIn vitro (cell-free)Mechanism
Enkephalinase from the striatum hydrolyzed VIP.
In plain wordsIn lab tests, an enzyme from brain striatum also broke down VIP.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableHydrolysis of VIP by striatum enkephalinase
Study
n: not reported in source
Enkephalinase from striatum membrane fraction
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
HDM and VIP were, however, hydrolyzed by the striatum enkephalinase.
1 context sentence(design, population, supporting numbers)
Summary · context
Degradation of HDM in the tracheal tissue isolated from a guinea-pig and by an isolated enkephalinase was studied and compared with the degradation of VIP.
Report a problemFND-0165263504
05LabIn vitro (cell-free)Mechanism
Thiorphan inhibited the enzymatic degradation of VIP.
In plain wordsIn lab tests, the enzyme blocker thiorphan reduced the breakdown of VIP.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
DecreaseInhibition of VIP degradation by thiorphan
Study
n: not reported in source
Purified enkephalinase
Outcome group
Receptor activity and mechanism · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
The degradation of VIP and HDM was inhibited by thiorphan.
1 context sentence(design, population, supporting numbers)
Summary · context
Degradation of HDM in the tracheal tissue isolated from a guinea-pig and by an isolated enkephalinase was studied and compared with the degradation of VIP.
Report a problemFND-0165263505

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
12 Oct 2026, 12:58 UTC
Study results updated
Version 1
9 Oct 2026, 12:41 UTC
First added · source update from Europe PMC

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