Common channels for water and protons at apical and basolateral cell membranes of frog skin and urinary bladder epithelia. Effects of oxytocin, heavy metals, and inhibitors of H(+)-adenosine triphosphatase.

Harvey B, Lacoste I, Ehrenfeld J

The Journal of general physiology1 Apr 1991Cited by 17 (Europe PMC)

PMID1647438PMCIDPMC2216497DOI10.1085/jgp.97.4.749PepBankPUB-01647438

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: CC BY-NC. Results still quote short attributed sentences.

Results from this study5

2 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueMechanism
In isolated frog skin, oxytocin treatment increased net titratable H+ flux from 30 to 115 neq.h-1.cm-2 at apical pH 5.5.
In plain wordsIn isolated frog skin, oxytocin increased the flow of protons across the tissue.
Peptide
Oxytocin
Outcome
IncreaseNet titratable H+ flux across frog skin
Study
frogn = 8
Isolated frog skin and urinary bladder epithelia
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Measurements
Net titratable H+ flux before oxytocin
30 neq.h-1.cm-2
apical pH 5.5, serosal pH 7.4
Net titratable H+ flux after oxytocin
115 neq.h-1.cm-2
apical pH 5.5, serosal pH 7.4
Source sentences
Summary
Net titratable H+ flux across frog skin was increased from 30 +/- 8 to 115 +/- 18 neq.h-1.cm-2 (n = 8) after oxytocin treatment (at apical pH 5.5 and serosal pH 7.4)
1 context sentence(design, population, supporting numbers)
Summary · context
We have compared the response of proton and water transport to oxytocin treatment in isolated frog skin and urinary bladder epithelia to provide further insights into the nature of water flow and H+ flux across individual apical and basolateral cell membranes.
Report a problemFND-0164743801
02LabEx vivo tissueMechanism
In frog skin principal cells, passive electrogenic H+ transport across the basolateral membrane was not affected by oxytocin.
In plain wordsIn isolated frog skin, oxytocin did not change proton movement across the lower cell membrane.
Peptide
Oxytocin
Outcome
No significant changeBasolateral passive H+ transport
Study
frogn: not reported in source
Isolated frog skin and urinary bladder epithelia
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
The basolateral membranes of the principal cells in frog skin epithelium were found to be spontaneously permeable to H+ and passive electrogenic H+ transport across this membrane was not affected by oxytocin.
1 context sentence(design, population, supporting numbers)
Summary · context
We have compared the response of proton and water transport to oxytocin treatment in isolated frog skin and urinary bladder epithelia to provide further insights into the nature of water flow and H+ flux across individual apical and basolateral cell membranes.
Report a problemFND-0164743802

Studies that did not test the peptide alone3

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

03LabNot a test of Oxytocin aloneEx vivo tissueMechanism
In frog skin exposed to oxytocin (50 mU/ml), lowering basolateral pH produced a transepithelial current that was blocked by Hg2+, Zn2+, Ni2+ or DCCD; oxytocin served as the condition, and the blockers were tested.
In plain wordsIn isolated frog skin, an acid-driven current seen with oxytocin present was blocked by several metals and a chemical inhibitor.
Peptide
Oxytocin
Outcome
Not applicableTransepithelial current at low basolateral pH and block by heavy metals
Study
frogn: not reported in source
Isolated frog skin and urinary bladder epithelia
Outcome group
Receptor activity and mechanism · Pharmacology
Measurements
Transepithelial current
3 microA.cm-2
pHb 5.5, oxytocin 50 mU/ml
Source sentences
Summary
Lowering pHb in the presence of oxytocin (50 mU/ml) produced a transepithelial current (3 microA.cm-2 at pHb 5.5) which was blocked by 100 microM of Hg2+, Zn2+, or Ni2+ at the basolateral side, and by DCCD (10(-5) M) or Hg2+ (100 microM) from the apical side.
1 context sentence(design, population, supporting numbers)
Summary · context
We have compared the response of proton and water transport to oxytocin treatment in isolated frog skin and urinary bladder epithelia to provide further insights into the nature of water flow and H+ flux across individual apical and basolateral cell membranes.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0164743803
04LabNot a test of Oxytocin aloneEx vivo tissueMechanism
In frog bladder sacs, the oxytocin-induced net hydroosmotic water flux was blocked by H+-ATPase inhibitors, including diethylstilbestrol, oligomycin and DCCD in the lumen.
In plain wordsIn frog bladder tissue, inhibitors of a proton pump stopped the water flow that oxytocin normally causes.
Peptide
Oxytocin
Outcome
DecreaseOxytocin-induced hydroosmotic water flux and H+-ATPase inhibitors
Study
frogn: not reported in source
Isolated frog skin and urinary bladder epithelia
Outcome group
Receptor activity and mechanism · Kidney and urinary
Source sentences
Summary
The net hydroosmotic water flux (JH2O) induced by oxytocin in frog bladder sacs was blocked by inhibitors of H(+)-adenosine triphosphatase (ATPase). Diethylstilbestrol (DES 10(-5) M), oligomycin (10(-8) M), and DCCD (10(-5) M) prevented JH2O when present in the lumen.
1 context sentence(design, population, supporting numbers)
Summary · context
We have compared the response of proton and water transport to oxytocin treatment in isolated frog skin and urinary bladder epithelia to provide further insights into the nature of water flow and H+ flux across individual apical and basolateral cell membranes.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0164743804
05LabNot a test of Oxytocin aloneEx vivo tissueMechanism
In frog bladder sacs, cyanide and 2-deoxyglucose had no effect on the water flux, so the inhibitor effects are not due to metabolic inhibition.
In plain wordsIn frog bladder tissue, two metabolic blockers did not change the water flow, so the proton pump inhibitors did not act through metabolism.
Peptide
Oxytocin
Outcome
No significant changeEffect of cyanide and 2-deoxyglucose on water flux
Study
frogn: not reported in source
Isolated frog skin and urinary bladder epithelia
Outcome group
Receptor activity and mechanism · Kidney and urinary
Source sentences
Summary
These effects cannot be attributed to inhibition of metabolism since cyanide (10(-4) M), or 2-deoxyglucose (10(-3) M) had no effect on JH2O.(ABSTRACT TRUNCATED AT 400 WORDS)
1 context sentence(design, population, supporting numbers)
Summary · context
We have compared the response of proton and water transport to oxytocin treatment in isolated frog skin and urinary bladder epithelia to provide further insights into the nature of water flow and H+ flux across individual apical and basolateral cell membranes.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0164743805

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
11 Oct 2026, 20:03 UTC
Study results updated
Version 1
9 Oct 2026, 02:52 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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