Acadesine and myocardial protection. Studies of time of administration and dose-response relations in the rat.

Galiñanes M, Mullane KM, Bullough D, Hearse DJ

Circulation1 Aug 1992Cited by 35 (Europe PMC)

PMID1638725DOI10.1161/01.cir.86.2.598PepBankPUB-01638725

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study16

16 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueEfficacy outcome
In isolated rat hearts, acadesine pretreatment alone increased aortic flow recovery versus acadesine-free control.
In plain wordsIn lab tests, acadesine pretreatment alone increased rat heart flow recovery compared with acadesine-free control.
Peptide
AICAR (acadesine)
Outcome
IncreaseAortic flow recovery, pretreatment only
Study
ratn = 24
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free control
Outcome group
Heart function · Cardiovascular
Studied dose
100 mg/kg · intravenous pretreatmentAs given in this study (rat).
Measurements
Aortic flow recovery
22.6 ml/min
pretreatment only
Aortic flow recovery
10.6 ml/min
control
Source sentences
Summary
Significant protection was observed in the first two groups (recovery of aortic flow increased from 10.6 +/- 2.6 ml/min in the acadesine-free control to 22.6 +/- 2.8 and 23.6 +/- 3.1 ml/min, respectively; p less than 0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
In studies (n = 12 per group) to determine whether acadesine was acting before, during, or after ischemia, the drug was given 1) only as pretreatment (100 mg/kg i.v.), 2) only during single-dose cardioplegia (20 mumol/l), or 3) only during reperfusion (20 mumol/l).
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872509
02LabEx vivo tissueEfficacy outcome
In isolated rat hearts, acadesine during cardioplegia alone increased aortic flow recovery versus acadesine-free control.
In plain wordsIn lab tests, acadesine in cardioplegia alone increased rat heart flow recovery compared with acadesine-free control.
Peptide
AICAR (acadesine)
Outcome
IncreaseAortic flow recovery, cardioplegia only
Study
ratn = 24
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free control
Outcome group
Heart function · Cardiovascular
Studied dose
20 mumol/l · Cardioplegic solutionAs given in this study (rat).
Measurements
Aortic flow recovery
23.6 ml/min
cardioplegia only
Aortic flow recovery
10.6 ml/min
control
Source sentences
Summary
Significant protection was observed in the first two groups (recovery of aortic flow increased from 10.6 +/- 2.6 ml/min in the acadesine-free control to 22.6 +/- 2.8 and 23.6 +/- 3.1 ml/min, respectively; p less than 0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
In studies (n = 12 per group) to determine whether acadesine was acting before, during, or after ischemia, the drug was given 1) only as pretreatment (100 mg/kg i.v.), 2) only during single-dose cardioplegia (20 mumol/l), or 3) only during reperfusion (20 mumol/l).
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872510
03LabEx vivo tissueEfficacy outcome
In isolated rat hearts, acadesine during reperfusion alone provided no significant protection versus acadesine-free control.
In plain wordsIn lab tests, acadesine during reperfusion alone did not significantly protect rat hearts compared with acadesine-free control.
Peptide
AICAR (acadesine)
Outcome
No significant changeProtection, reperfusion only
Study
ratn = 24
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free control
Outcome group
Heart function · Cardiovascular
Studied dose
20 mumol/l · Reperfusion solutionAs given in this study (rat).
Source sentences
Summary
No significant protection was observed when acadesine was given only during reperfusion.
2 context sentences(design, population, supporting numbers)
Summary · context
In studies (n = 12 per group) to determine whether acadesine was acting before, during, or after ischemia, the drug was given 1) only as pretreatment (100 mg/kg i.v.), 2) only during single-dose cardioplegia (20 mumol/l), or 3) only during reperfusion (20 mumol/l).
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872511
04LabEx vivo tissueEfficacy outcome
In isolated rat hearts, aortic flow recovery was numerically higher with 5 mumol/l acadesine than control (16.9 versus 15.4 ml/min).
In plain wordsIn lab tests, rat heart flow recovery was numerically higher with 5 mumol/l acadesine than control (16.9 versus 15.4 ml/min).
Peptide
AICAR (acadesine)
Outcome
IncreaseAortic flow recovery, low concentration
Study
ratn = 24
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free cardioplegia
Outcome group
Heart function · Cardiovascular
Studied dose
5 mumol/l · Cardioplegic solutionAs given in this study (rat).
Measurements
Aortic flow recovery
16.9 ml/min
5 mumol/l acadesine
Reported uncertainty
3.6 ml/min
type not stated
Source sentences
Summary
In dose-response studies, acadesine (0, 5, 20, 50, 200, and 1,000 mumol/l; n = 12 per group) was given only as a cardioplegic additive; the postischemic recoveries of aortic flow were 15.4 +/- 2.8, 16.9 +/- 3.6, 29.5 +/- 3.8, 27.4 +/- 3.8, 26.7 +/- 4.2, and 27.1 +/- 2.7 ml/min, respectively.
1 context sentence(design, population, supporting numbers)
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872512
05LabEx vivo tissueEfficacy outcome
In isolated rat hearts, aortic flow recovery was numerically higher with 20 mumol/l acadesine than control (29.5 versus 15.4 ml/min).
In plain wordsIn lab tests, rat heart flow recovery was numerically higher with 20 mumol/l acadesine than control (29.5 versus 15.4 ml/min).
Peptide
AICAR (acadesine)
Outcome
IncreaseAortic flow recovery, cardioplegia concentration
Study
ratn = 24
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free cardioplegia
Outcome group
Heart function · Cardiovascular
Studied dose
20 mumol/l · Cardioplegic solutionAs given in this study (rat).
Measurements
Aortic flow recovery
29.5 ml/min
20 mumol/l acadesine
Reported uncertainty
3.8 ml/min
type not stated
Source sentences
Summary
In dose-response studies, acadesine (0, 5, 20, 50, 200, and 1,000 mumol/l; n = 12 per group) was given only as a cardioplegic additive; the postischemic recoveries of aortic flow were 15.4 +/- 2.8, 16.9 +/- 3.6, 29.5 +/- 3.8, 27.4 +/- 3.8, 26.7 +/- 4.2, and 27.1 +/- 2.7 ml/min, respectively.
1 context sentence(design, population, supporting numbers)
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872513
06LabEx vivo tissueEfficacy outcome
In isolated rat hearts, aortic flow recovery was numerically higher with 50 mumol/l acadesine than control (27.4 versus 15.4 ml/min).
In plain wordsIn lab tests, rat heart flow recovery was numerically higher with 50 mumol/l acadesine than control (27.4 versus 15.4 ml/min).
Peptide
AICAR (acadesine)
Outcome
IncreaseAortic flow recovery, cardioplegia concentration
Study
ratn = 24
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free cardioplegia
Outcome group
Heart function · Cardiovascular
Studied dose
50 mumol/l · Cardioplegic solutionAs given in this study (rat).
Measurements
Aortic flow recovery
27.4 ml/min
50 mumol/l acadesine
Reported uncertainty
3.8 ml/min
type not stated
Source sentences
Summary
In dose-response studies, acadesine (0, 5, 20, 50, 200, and 1,000 mumol/l; n = 12 per group) was given only as a cardioplegic additive; the postischemic recoveries of aortic flow were 15.4 +/- 2.8, 16.9 +/- 3.6, 29.5 +/- 3.8, 27.4 +/- 3.8, 26.7 +/- 4.2, and 27.1 +/- 2.7 ml/min, respectively.
1 context sentence(design, population, supporting numbers)
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872514
07LabEx vivo tissueEfficacy outcome
In isolated rat hearts, aortic flow recovery was numerically higher with 200 mumol/l acadesine than control (26.7 versus 15.4 ml/min).
In plain wordsIn lab tests, rat heart flow recovery was numerically higher with 200 mumol/l acadesine than control (26.7 versus 15.4 ml/min).
Peptide
AICAR (acadesine)
Outcome
IncreaseAortic flow recovery, cardioplegia concentration
Study
ratn = 24
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free cardioplegia
Outcome group
Heart function · Cardiovascular
Studied dose
200 mumol/l · Cardioplegic solutionAs given in this study (rat).
Measurements
Aortic flow recovery
26.7 ml/min
200 mumol/l acadesine
Reported uncertainty
4.2 ml/min
type not stated
Source sentences
Summary
In dose-response studies, acadesine (0, 5, 20, 50, 200, and 1,000 mumol/l; n = 12 per group) was given only as a cardioplegic additive; the postischemic recoveries of aortic flow were 15.4 +/- 2.8, 16.9 +/- 3.6, 29.5 +/- 3.8, 27.4 +/- 3.8, 26.7 +/- 4.2, and 27.1 +/- 2.7 ml/min, respectively.
1 context sentence(design, population, supporting numbers)
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872515
08LabEx vivo tissueEfficacy outcome
In isolated rat hearts, aortic flow recovery was numerically higher with 1,000 mumol/l acadesine than control (27.1 versus 15.4 ml/min).
In plain wordsIn lab tests, rat heart flow recovery was numerically higher with 1,000 mumol/l acadesine than control (27.1 versus 15.4 ml/min).
Peptide
AICAR (acadesine)
Outcome
IncreaseAortic flow recovery, highest concentration
Study
ratn = 24
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free cardioplegia
Outcome group
Heart function · Cardiovascular
Studied dose
1,000 mumol/l · Cardioplegic solutionAs given in this study (rat).
Measurements
Aortic flow recovery
27.1 ml/min
1,000 mumol/l acadesine
Reported uncertainty
2.7 ml/min
type not stated
Source sentences
Summary
In dose-response studies, acadesine (0, 5, 20, 50, 200, and 1,000 mumol/l; n = 12 per group) was given only as a cardioplegic additive; the postischemic recoveries of aortic flow were 15.4 +/- 2.8, 16.9 +/- 3.6, 29.5 +/- 3.8, 27.4 +/- 3.8, 26.7 +/- 4.2, and 27.1 +/- 2.7 ml/min, respectively.
1 context sentence(design, population, supporting numbers)
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872516
09LabEx vivo tissueEfficacy outcome
In isolated rat hearts, combined acadesine pretreatment, cardioplegia, and reperfusion improved aortic flow recovery versus drug-free control.
In plain wordsIn lab tests, acadesine before ischemia, in cardioplegia, and during reperfusion improved rat heart flow recovery compared with drug-free control.
Peptide
AICAR (acadesine)
Outcome
IncreaseAortic flow recovery, combined administration
Study
ratn = 16
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free control
Outcome group
Heart function · Cardiovascular
Studied dose
100 mg/kg pretreatment; 20 mumol/l cardioplegia and initial reperfusion · Intravenous pretreatment and perfusion solutions · 15 minutes before study and cardioplegia and initial 15 minutes of reperfusionAs given in this study (rat).
Measurements
Aortic flow recovery
28.9 ml/min
acadesine after 2.5 hours ischemia
Aortic flow recovery
16.5 ml/min
control after 2.5 hours ischemia
Source sentences
Summary
the recovery of aortic flow after 2.5 hours of ischemia was improved from its control value of 16.5 +/- 3.9 ml/min to 28.9 +/- 4.1 ml/min (n = 8 per group; p less than 0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
When acadesine was given as an in vivo pretreatment (100 mg/kg i.v. 15 minutes before study) followed by being administered as an additive (20 mumol/l) to the St. Thomas' Hospital cardioplegic solution (single dose) and then as an additive (20 mumol/l) to the initial reperfusion (15 minutes) solution
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872501
10LabEx vivo tissueEfficacy outcome
In isolated rat hearts, combined acadesine administration similarly protected other unspecified cardiac function indexes versus drug-free control.
In plain wordsIn lab tests, combined acadesine administration also protected other unspecified rat heart function measures compared with drug-free control.
Peptide
AICAR (acadesine)
Outcome
IncreaseOther cardiac function indexes, combined administration
Study
ratn = 16
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free control
Outcome group
Heart function · Cardiovascular
Studied dose
100 mg/kg pretreatment; 20 mumol/l cardioplegia and initial reperfusion · Intravenous pretreatment and perfusion solutionsAs given in this study (rat).
Source sentences
Summary
Similar protection was seen with other indexes of cardiac function.
2 context sentences(design, population, supporting numbers)
Summary · context
the recovery of aortic flow after 2.5 hours of ischemia was improved from its control value of 16.5 +/- 3.9 ml/min to 28.9 +/- 4.1 ml/min (n = 8 per group; p less than 0.05).
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872502
11LabEx vivo tissueEfficacy outcome
In isolated rat hearts with prolonged ischemia and multidose cardioplegia, acadesine increased mean functional recovery versus control.
In plain wordsIn lab tests, acadesine improved rat heart function recovery after prolonged ischemia and repeated cardioplegia compared with control.
Peptide
AICAR (acadesine)
Outcome
IncreaseFunctional recovery, multidose cardioplegia
Study
ratn = 16
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free control
Outcome group
Heart function · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
In further studies (n = 8 per group), with multidose (every 30 minutes) cardioplegia and extended periods (6 hours) of hypothermic ischemia, acadesine consistently led to higher mean recoveries of function and lower levels of creatine kinase leakage.
1 context sentence(design, population, supporting numbers)
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872505
12LabEx vivo tissueEfficacy outcome
In isolated rat hearts with prolonged ischemia and multidose cardioplegia, acadesine reduced creatine kinase leakage versus control.
In plain wordsIn lab tests, acadesine reduced creatine kinase leakage from rat hearts after prolonged ischemia and repeated cardioplegia compared with control.
Peptide
AICAR (acadesine)
Outcome
DecreaseCreatine kinase leakage, multidose cardioplegia
Study
ratn = 16
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free control
Outcome group
Heart function · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
In further studies (n = 8 per group), with multidose (every 30 minutes) cardioplegia and extended periods (6 hours) of hypothermic ischemia, acadesine consistently led to higher mean recoveries of function and lower levels of creatine kinase leakage.
1 context sentence(design, population, supporting numbers)
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872506
13LabEx vivo tissueMechanism
In isolated rat hearts with prolonged ischemia and multidose cardioplegia, acadesine increased tissue inosine monophosphate content versus control.
In plain wordsIn lab tests, acadesine increased rat heart inosine monophosphate after prolonged ischemia and repeated cardioplegia compared with control.
Peptide
AICAR (acadesine)
Outcome
IncreaseInosine monophosphate content, multidose cardioplegia
Study
ratn = 16
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free control
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
Again, the only significant metabolic effect was an increase in tissue inosine monophosphate content.
2 context sentences(design, population, supporting numbers)
Summary · context
In further studies (n = 8 per group), with multidose (every 30 minutes) cardioplegia and extended periods (6 hours) of hypothermic ischemia, acadesine consistently led to higher mean recoveries of function and lower levels of creatine kinase leakage.
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872507
14LabEx vivo tissueMechanism
In isolated rat hearts with multidose cardioplegia, acadesine showed no significant metabolic effect versus control apart from increased inosine monophosphate.
In plain wordsIn lab tests, acadesine showed no significant rat heart metabolic effect versus control after repeated cardioplegia, except for increased inosine monophosphate.
Peptide
AICAR (acadesine)
Outcome
No significant changeOther metabolites, multidose cardioplegia
Study
ratn = 16
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free control
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
Again, the only significant metabolic effect was an increase in tissue inosine monophosphate content.
2 context sentences(design, population, supporting numbers)
Summary · context
In further studies (n = 8 per group), with multidose (every 30 minutes) cardioplegia and extended periods (6 hours) of hypothermic ischemia, acadesine consistently led to higher mean recoveries of function and lower levels of creatine kinase leakage.
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872508
15LabEx vivo tissueMechanism
In isolated rat hearts, acadesine did not significantly change metabolite content versus control, except for increased inosine monophosphate.
In plain wordsIn lab tests, acadesine did not significantly change rat heart metabolite levels compared with control, except for increased inosine monophosphate.
Peptide
AICAR (acadesine)
Outcome
No significant changeOther metabolite content after ischemia and reperfusion
Study
ratn: not reported in source
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free control
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
100 mg/kg pretreatment; 20 mumol/l cardioplegia and initial reperfusion · Intravenous pretreatment and perfusion solutionsAs given in this study (rat).
Source sentences
Summary
Analysis of hearts obtained at the end of 2.5 hours of ischemia and 35 minutes of reperfusion revealed no significant differences in metabolite content between control and drug-treated hearts with the exception of inosine monophosphate, which was increased from its drug-free control value of 0.10 +/…
1 context sentence(design, population, supporting numbers)
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872503
16LabEx vivo tissueMechanism
In isolated rat hearts, combined acadesine administration increased inosine monophosphate content versus drug-free control after ischemia and reperfusion.
In plain wordsIn lab tests, combined acadesine administration increased rat heart inosine monophosphate levels compared with drug-free control after ischemia and reperfusion.
Peptide
AICAR (acadesine)
Outcome
IncreaseInosine monophosphate content, combined administration
Study
ratn: not reported in source
Isolated perfused rat heart with hypothermic ischemia and reperfusion
Comparator
Acadesine-free control
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
100 mg/kg pretreatment; 20 mumol/l cardioplegia and initial reperfusion · Intravenous pretreatment and perfusion solutionsAs given in this study (rat).
Measurements
Inosine monophosphate content
0.86 mumol/g dry wt
acadesine
Inosine monophosphate content
0.1 mumol/g dry wt
control
Source sentences
Summary
Analysis of hearts obtained at the end of 2.5 hours of ischemia and 35 minutes of reperfusion revealed no significant differences in metabolite content between control and drug-treated hearts with the exception of inosine monophosphate, which was increased from its drug-free control value of 0.10 +/…
1 context sentence(design, population, supporting numbers)
Summary · context
The protective properties of acadesine were studied in the isolated, perfused rat heart subjected to global hypothermic (20 degrees C) ischemia and reperfusion.
Report a problemFND-0163872504

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

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