In cultured bovine granulosa cells, vasoactive intestinal peptide at 2.5-1000 ng/ml for two days had no effect on proliferation, with or without FSH.
In plain wordsIn cells from cows, VIP did not change granulosa cell growth, with or without FSH.
- Outcome
- No significant changeGranulosa cell proliferation
- Study
bovinen: not reported in source
Cultured bovine granulosa cells- Outcome group
- Cell proliferation and differentiation · Reproductive and sexual health
- Studied dose
- 2.5-1000 ng/ml · 2 daysAs given in this study (bovine).
Source sentences
Two-day treatment with 2.5-1000 ng/ml of VIP had no effect (P greater than 0.05) on proliferation or progesterone production of bovine GC in the presence or absence of 200 ng/ml FSH.
1 context sentence(design, population, supporting numbers)
Undifferentiated GC from 1-5 mm bovine follicles were established for 2 days in medium containing 10% fetal calf serum, washed and then cultured in chemically defined medium for an additional 2 days.