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Study · Not a test of the peptide alone · 1992

5 results

Semisynthesis of human growth hormone-releasing factors by alpha-amidating enzyme catalyzed oxidation of glycine-extended precursors.

Bongers J, Felix AM, Campbell RM, Lee Y, Merkler DJ, Heimer EP

Peptide research1 Jul 1992Cited by 7 (Europe PMC)

PMID1421807 (opens in a new tab)PepBankPUB-01421807
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, Sermorelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Sermorelin.

  1. 01LabNot a test of Sermorelin aloneIn vitro (cells)Activity measurement

    The semisynthetic products each possessed full potency in an in vitro rat pituitary bioassay.

    In plain wordsIn lab tests with rat pituitary cells, the made peptides were as potent as expected.

    Peptide
    Sermorelin
    Outcome
    Not applicablePotency of semisynthetic products
    Study

    ratn: not reported in source

    in vitro rat pituitary bioassay
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    the products each possessed full potency in an in vitro rat pituitary bioassay

    In this study, Sermorelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Sermorelin.

  2. 02LabNot a test of Sermorelin aloneIn vitro (cell-free)Other

    Semisynthesis by alpha-amidating enzyme oxidation of glycine-extended precursors gave isolated yields of about 75%.

    In plain wordsIn lab chemistry, an enzyme step turned the precursor peptides into the final peptides with a yield of about 75%.

    Peptide
    Sermorelin
    Outcome
    Not applicableIsolated yield of semisynthesis
    Study

    n: not reported in source

    enzymatic semisynthesis with alpha-amidating enzyme
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    Isolated yield
    ≈ 75%
    semisynthesis products

    Source sentences

    Summary
    Isolated yields of about 75% were obtained

    In this study, Sermorelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Sermorelin.

  3. 03LabNot a test of Sermorelin aloneIn vitro (cell-free)Activity measurement

    The semisynthetic products each possessed full receptor-binding affinity.

    In plain wordsIn lab tests, the made peptides bound to the receptor as strongly as expected.

    Peptide
    Sermorelin
    Outcome
    Not applicableReceptor-binding affinity of semisynthetic products
    Study

    n: not reported in source

    receptor binding assay
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    and full receptor-binding affinity.

    In this study, Sermorelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Sermorelin.

  4. 04LabNot a test of Sermorelin aloneIn vitro (cell-free)Other

    A transient alpha-hydroxyglycine intermediate, [Ala15]-GRF(1-29)-Gly(alpha-OH)-OH, was isolated and characterized.

    In plain wordsIn lab chemistry, a short-lived intermediate of the enzyme reaction was isolated and described.

    Peptide
    Sermorelin
    Outcome
    Not applicableAlpha-hydroxyglycine intermediate
    Study

    n: not reported in source

    enzymatic semisynthesis with alpha-amidating enzyme
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    A transient alpha-hydroxyglycine intermediate, [Ala15]-GRF(1-29)-Gly(alpha-OH)-OH, was isolated and characterized.

    In this study, Sermorelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Sermorelin.

  5. 05LabNot a test of Sermorelin aloneIn vitro (cell-free)Other

    Kinetic studies showed that the rat alpha-amidating enzyme from recombinant mouse C127 cells has both monooxygenase and lyase activities needed for both amidation steps.

    In plain wordsIn lab chemistry, the enzyme carried out both steps needed to add the amide group to the peptide.

    Peptide
    Sermorelin
    Outcome
    Not applicableAlpha-amidating enzyme activities
    Study

    n: not reported in source

    recombinant rat alpha-amidating enzyme
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    Kinetic studies with this intermediate demonstrate that the rat alpha-amidating enzyme from recombinant mouse C127 cells possesses both the monooxygenase and lyase activities needed to catalyze both steps of the amidation process.

    In this study, Sermorelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Sermorelin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 10:21 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:13 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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