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Study · Ex vivo tissue · rat · 1992

6 results

Regional distribution of guanine nucleotide-sensitive and guanine nucleotide-insensitive vasoactive intestinal peptide receptors in rat brain.

Hill JM, Harris A, Hilton-Clarke DI

Neuroscience1 Jun 1992Cited by 37 (Europe PMC)

PMID1321366 (opens in a new tab)DOI10.1016/0306-4522(92)90280-f (opens in a new tab)PepBankPUB-01321366
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueActivity measurement

    In rat brain sections, guanylyl-imidodiphosphate reduced VIP binding by 40 to 60% in most brain regions.

    In plain wordsIn rat brain tissue, a GTP-like compound reduced VIP binding by 40 to 60% in most regions.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    DecreaseVIP binding with guanylyl-imidodiphosphate in most brain regions
    Study

    ratn: not reported in source

    Rat brain sections (in vitro autoradiography)
    Comparator
    VIP binding without guanylyl-imidodiphosphate
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In most brain regions, guanylyl-imidodiphosphate reduced vasoactive intestinal peptide binding between 40 and 60%.
    1 context sentence
    Summary · context
    Based on the ability of guanine nucleotides to inhibit the binding of vasoactive intestinal peptide to its receptors, a guanosine 5'-triphosphate analog, guanylyl-imidodiphosphate, was used to differentiate two subtypes (or different functional states of a single subtype) of vasoactive intestinal pe…
  2. 02LabEx vivo tissueActivity measurement

    In rat brain sections, guanylyl-imidodiphosphate inhibited 80% or more of VIP binding in the supraoptic nucleus, locus coeruleus, interpeduncular nucleus, facial nucleus, olfactory tubercle and periventricular hypothalamic nucleus.

    In plain wordsIn rat brain tissue, a GTP-like compound blocked 80% or more of VIP binding in six regions, including the locus coeruleus.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    DecreaseVIP binding with guanylyl-imidodiphosphate in supraoptic nucleus, locus coeruleus and other regions
    Study

    ratn: not reported in source

    Rat brain sections (in vitro autoradiography)
    Comparator
    VIP binding without guanylyl-imidodiphosphate
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    However, in the supraoptic nucleus, locus coeruleus, interpeduncular nucleus, facial nucleus, olfactory tubercle and periventricular hypothalamic nucleus, 80% or more of vasoactive intestinal peptide binding was inhibited.
    1 context sentence
    Summary · context
    Based on the ability of guanine nucleotides to inhibit the binding of vasoactive intestinal peptide to its receptors, a guanosine 5'-triphosphate analog, guanylyl-imidodiphosphate, was used to differentiate two subtypes (or different functional states of a single subtype) of vasoactive intestinal pe…
  3. 03LabEx vivo tissueActivity measurement

    In rat brain sections, guanylyl-imidodiphosphate had little effect on VIP binding in the medial geniculate, olfactory bulbs and ventral thalamic nuclei.

    In plain wordsIn rat brain tissue, a GTP-like compound had little effect on VIP binding in the medial geniculate, olfactory bulbs and ventral thalamus.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    No significant changeVIP binding with guanylyl-imidodiphosphate in medial geniculate, olfactory bulbs and ventral thalamic nuclei
    Study

    ratn: not reported in source

    Rat brain sections (in vitro autoradiography)
    Comparator
    VIP binding without guanylyl-imidodiphosphate
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In other brain regions, including the medial geniculate, olfactory bulbs, and ventral thalamic nuclei, guanylyl-imidodiphosphate had little effect on vasoactive intestinal peptide binding.
    1 context sentence
    Summary · context
    Based on the ability of guanine nucleotides to inhibit the binding of vasoactive intestinal peptide to its receptors, a guanosine 5'-triphosphate analog, guanylyl-imidodiphosphate, was used to differentiate two subtypes (or different functional states of a single subtype) of vasoactive intestinal pe…
  4. 04LabEx vivo tissueActivity measurement

    In rat liver, lung and intestine, guanylyl-imidodiphosphate partly inhibited VIP binding.

    In plain wordsIn rat liver, lung and gut tissue, a GTP-like compound partly reduced VIP binding.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    DecreaseVIP binding with guanylyl-imidodiphosphate in liver, lung and intestine
    Study

    ratn: not reported in source

    Rat tissue sections (in vitro autoradiography)
    Comparator
    VIP binding without guanylyl-imidodiphosphate
    Outcome group
    Receptor activity and mechanism · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In liver, lung and intestine it also partly inhibited vasoactive intestinal peptide binding.
    1 context sentence
    Summary · context
    Based on the ability of guanine nucleotides to inhibit the binding of vasoactive intestinal peptide to its receptors, a guanosine 5'-triphosphate analog, guanylyl-imidodiphosphate, was used to differentiate two subtypes (or different functional states of a single subtype) of vasoactive intestinal pe…
  5. 05LabEx vivo tissueActivity measurement

    In rat brain membranes, VIP cross-linked to its receptors gave a pair of bands between 44,000 and 52,000 mol. wt, and components at 64,000 and 92,000 mol. wt.

    In plain wordsIn rat brain membranes, VIP attached to four receptor-related protein bands of different sizes.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableMolecular weights of cross-linked VIP receptor bands in brain membranes
    Study

    ratn: not reported in source

    Rat brain sections (in vitro autoradiography)
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Electrophoretic analysis of vasoactive intestinal peptide, covalently cross-linked to its receptors in brain membranes, revealed a pair of bands between 44,000 and 52,000 mol. wt, a component at 64,000 mol. wt and another at 92,000 mol. wt.
    1 context sentence
    Summary · context
    Based on the ability of guanine nucleotides to inhibit the binding of vasoactive intestinal peptide to its receptors, a guanosine 5'-triphosphate analog, guanylyl-imidodiphosphate, was used to differentiate two subtypes (or different functional states of a single subtype) of vasoactive intestinal pe…
  6. 06LabEx vivo tissueActivity measurement

    In rat brain membranes, all cross-linked bands were displaceable with VIP but guanylyl-imidodiphosphate displaced only the 64,000 mol. wt band, suggesting a GTP-sensitive VIP receptor of about 61,000 mol. wt.

    In plain wordsIn rat brain membranes, a GTP-like compound displaced VIP from only one receptor band, pointing to a GTP-sensitive VIP receptor subtype.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableGTP-sensitive VIP receptor band
    Study

    ratn: not reported in source

    Rat brain sections (in vitro autoradiography)
    Comparator
    VIP binding without guanylyl-imidodiphosphate
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    All were displaceable with vasoactive intestinal peptide but guanylyl-imidodiphosphate displaced only the 64,000 mol. wt band suggesting that the GTP-sensitive vasoactive intestinal peptide receptor seen in brain sections has a molecular weight of about 61,000.
    1 context sentence
    Summary · context
    Based on the ability of guanine nucleotides to inhibit the binding of vasoactive intestinal peptide to its receptors, a guanosine 5'-triphosphate analog, guanylyl-imidodiphosphate, was used to differentiate two subtypes (or different functional states of a single subtype) of vasoactive intestinal pe…

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    12 Oct 2026, 12:58 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 12:41 UTC

    First added · source update from Europe PMC

Subjects

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