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Study · In vitro (cells) · 1992

4 results

Processing of vasoactive intestinal peptide and transferrin in human cancerous colonic cells.

Phan HH, Barakat A, Lefevre C, Boissard C, Rosselin G

Peptides1 Jan 1992Cited by 6 (Europe PMC)

PMID1320267 (opens in a new tab)DOI10.1016/0196-9781(92)90139-t (opens in a new tab)PepBankPUB-01320267
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In HT-29 human colon cancer cells, potassium depletion inhibited VIP internalization by 23%.

    In plain wordsIn cells, lowering potassium reduced VIP uptake by 23%, which points to a role for clathrin-coated pits.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    DecreaseVIP internalization after potassium depletion
    Study

    humann: not reported in source

    HT-29 human colon cancer cells
    Comparator
    Cells without potassium depletion
    Outcome group
    Receptor activity and mechanism · Cancer
    Studied dose
    Dose not stated

    Measurements

    VIP internalization inhibition
    23%
    potassium-depleted HT-29 cells

    Source sentences

    Summary
    Cellular depletion in potassium inhibits the internalization of VIP (23%) and to a greater extent (42%) that of Tf.
    2 context sentences
    Summary · context
    This indicates that clathrin-coated pits are also involved, at least in part, in VIP uptake.
    Summary · context
    Endocytosis of vasoactive intestinal peptide (VIP) and of transferrin (Tf) was comparatively studied in human cancerous colonic HT-29 cells.
  2. 02LabIn vitro (cells)Mechanism

    In HT-29 cells, vesicles containing 125I-VIP separated into low and high density subpopulations on sucrose gradients.

    In plain wordsIn cells, vesicles that carry VIP split into a lighter group and a denser group.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableVesicle subpopulations containing VIP
    Study

    humann: not reported in source

    HT-29 human colon cancer cells
    Outcome group
    Receptor activity and mechanism · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The distribution of 125I-Tf- or 125I-VIP-containing vesicles in sucrose gradients revealed low and high density vesicle subpopulations.
    1 context sentence
    Summary · context
    Endocytosis of vasoactive intestinal peptide (VIP) and of transferrin (Tf) was comparatively studied in human cancerous colonic HT-29 cells.
  3. 03LabIn vitro (cells)Mechanism

    In HT-29 cells, the low density vesicle compartment was transient: some vesicles recycled to the membrane, while those with ligand mostly moved to the high density compartment and fused with heavy vesicles.

    In plain wordsIn cells, VIP-carrying vesicles moved from a short-lived light compartment to a denser one, and some content was recycled to the cell surface.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableIntracellular trafficking of VIP ligand between vesicle compartments
    Study

    humann: not reported in source

    HT-29 human colon cancer cells
    Outcome group
    Receptor activity and mechanism · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The low density vesicle subpopulation represented a transient compartment from which incoming vesicles containing N-leucyl-beta naphthylamidase were recycled back to the membrane
    2 context sentences
    Summary · context
    Subsequent fusion of the latter with heavy vesicles
    Summary · context
    Endocytosis of vasoactive intestinal peptide (VIP) and of transferrin (Tf) was comparatively studied in human cancerous colonic HT-29 cells.
  4. 04LabIn vitro (cells)Mechanism

    In HT-29 cells, two-thirds of VIP shifted with transferrin-containing vesicles to denser fractions and the remainder was found with unshifted vesicles, so the VIP-receptor complex follows two routes.

    In plain wordsIn cells, VIP and its receptor are processed by two routes, and the main route is shared with transferrin uptake.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableRoutes of VIP-receptor complex processing
    Study

    humann: not reported in source

    HT-29 human colon cancer cells
    Outcome group
    Receptor activity and mechanism · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    two-thirds of VIP shifted with the Tf-HRP-containing vesicles to denser fractions and the remaining was found with unshifted vesicles
    2 context sentences
    Summary · context
    These findings indicate that the VIP-receptor complex processing in HT-29 cells follows two routes, the major route being common with Tf endocytosis.
    Summary · context
    Endocytosis of vasoactive intestinal peptide (VIP) and of transferrin (Tf) was comparatively studied in human cancerous colonic HT-29 cells.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    12 Oct 2026, 12:58 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 12:41 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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