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Study · In vitro (cells) · mouse and rat · 1992

4 results

Preparation and characterization of a mouse osteoclast-like multinucleated cell population.

Akatsu T, Tamura T, Takahashi N, Udagawa N, Tanaka S, Sasaki T, Yamaguchi A, Nagata N, Suda T

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research1 Nov 1992Cited by 124 (Europe PMC)

PMID1281605 (opens in a new tab)DOI10.1002/jbmr.5650071109 (opens in a new tab)PepBankPUB-01281605
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In mouse osteoclast-like multinucleated cells, salmon calcitonin increased cAMP, with maximal response at 10(-10) M and ED50 2.2 x 10(-11) M.

    In plain wordsIn mouse cells, salmon calcitonin increased cAMP production.

    Peptide
    Calcitonin-salmon
    Outcome
    IncreasecAMP production
    Study

    mousen: not reported in source

    Osteoclast-like multinucleated cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    10(-10) M maximal response; ED50 2.2 x 10(-11) MAs given in this study (mouse).

    Source sentences

    Summary
    Salmon and human CT increased cAMP production (maximal response: slmon CT at 10(-10) M, human CT at 10(-8) M; ED50s: salmon CT, 2.2 x 10(-11) M, human CT, 1.3 x 10(-9) M) in the MNC preparation.
    1 context sentence
    Summary · context
    TRAP+ MNCs were formed in cocultures of mouse osteoblastic cells and marrow cells on 10 cm collagen gel-coated dishes.
  2. 02LabIn vitro (cells)Activity measurement

    In mouse osteoclast-like multinucleated cells, radiolabeled salmon calcitonin had a Kd of 8.9 +/- 0.7 x 10(-10) M.

    In plain wordsIn mouse cells, laboratory tests measured salmon calcitonin binding.

    Peptide
    Calcitonin-salmon
    Outcome
    Not applicableBinding affinity
    Study

    mousen: not reported in source

    Osteoclast-like multinucleated cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Saturation analysis of [125I]salmon CT indicated a dissociation constant Kd for TRAP+ MNCs of 8.9 +/- 0.7 x 10(-10) M and 16.5 +/- 1.5 x 10(6) binding sites per cell.
    1 context sentence
    Summary · context
    TRAP+ MNCs were formed in cocultures of mouse osteoblastic cells and marrow cells on 10 cm collagen gel-coated dishes.
  3. 03LabIn vitro (cells)Activity measurement

    In mouse osteoclast-like multinucleated cells, radiolabeled salmon calcitonin had 16.5 +/- 1.5 x 10(6) binding sites per cell.

    In plain wordsIn mouse cells, laboratory tests measured salmon calcitonin binding.

    Peptide
    Calcitonin-salmon
    Outcome
    Not applicableBinding sites
    Study

    mousen: not reported in source

    Osteoclast-like multinucleated cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Saturation analysis of [125I]salmon CT indicated a dissociation constant Kd for TRAP+ MNCs of 8.9 +/- 0.7 x 10(-10) M and 16.5 +/- 1.5 x 10(6) binding sites per cell.
    1 context sentence
    Summary · context
    TRAP+ MNCs were formed in cocultures of mouse osteoblastic cells and marrow cells on 10 cm collagen gel-coated dishes.
  4. 04LabIn vitro (cells)Activity measurement

    In mouse MNC and rat OCL preparations, displacement curves for radiolabeled salmon calcitonin with unlabeled salmon calcitonin were similar.

    In plain wordsIn mouse and rat cell preparations, salmon calcitonin displacement curves were similar.

    Peptide
    Calcitonin-salmon
    Outcome
    Direction not reportedCalcitonin displacement curves
    Study

    mouse and ratn: not reported in source

    MNC and isolated osteoclast preparations
    Comparator
    Rat OCL preparations
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Displacement curves for [125I]salmon CT with unlabeled salmon and human CT were similar in MNC and OCL preparations.
    2 context sentences
    Summary · context
    TRAP+ MNCs were formed in cocultures of mouse osteoblastic cells and marrow cells on 10 cm collagen gel-coated dishes.
    Summary · context
    These results were similar to the Kd value (3.5 x 10(-10) M) and the number of binding sites (3.3 x 10(6) per cell) in isolated rat OCLs.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    28 Oct 2026, 02:03 UTC

    Study results updated

  2. Version 1

    27 Oct 2026, 16:35 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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