The hypothalamic-neurohypophysial system of the rat: localization and quantitation of neurophysin by light microscopic immunocytochemistry in normal rats and in Brattleboro rats deficient in vasopressin and a neurophysin.

Sokol HW, Zimmerman EA, Sawyer WH, Robinson AG

Endocrinology1 May 1976Cited by 92 (Europe PMC)

PMID1261512DOI10.1210/endo-98-5-1176PepBankPUB-01261512

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

No result from this study comes from a test of the peptide.

Natural levels measured6

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01LabEndogenous levelEx vivo tissueMechanism
In homozygous Brattleboro rats with diabetes insipidus, about half of supraoptic and paraventricular magnocellular neurons lacked oxytocin immunoreactivity.
In plain wordsIn lab tests of homozygous Brattleboro rat tissue, about half of key brain neurons lacked staining for the body's own oxytocin.
Peptide
Oxytocin
Outcome
Not applicableOxytocin immunoreactivity in homozygous Brattleboro magnocellular neurons
Study
ratn: not reported in source
hypothalamic and neural lobe tissue sections
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
About half of the magnocellular neurons in the supraoptic (SON) and paraventricular (PVN) nuclei of homozygous Brattleboro rats with diabetes insipidus (DI) were devoid of immunoreactive neurophysin, OT, and VP.
3 context sentences(design, population, supporting numbers)
Summary · context
The cellular distribution of neurophysin was examined in hypothalami and neural lobes of normal Long-Evans rats and Brattleboro rats deficient in vasopressin and a major neurophysin.
Summary · context
Tissue sections were treated with antisera to bovine, human, and rat neurophysins, using immunoperoxidase bridge techniques.
Summary · context
Antisera to oxytocin (OT) and vasopressin (VP) were applied to adjacent sections.
This result measures the body's own Oxytocin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0126151201
02LabEndogenous levelEx vivo tissueMechanism
In homozygous Brattleboro rats, cells immunoreactive for oxytocin were more concentrated in the dorsal supraoptic nucleus and peripheral paraventricular nucleus.
In plain wordsIn lab tests of homozygous Brattleboro rat tissue, cells immunoreactive for the body's own oxytocin were concentrated in particular hypothalamic regions.
Peptide
Oxytocin
Outcome
Not applicableDistribution of oxytocin-immunoreactive cells in homozygous Brattleboro rats
Study
ratn: not reported in source
hypothalamic and neural lobe tissue sections
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
The cells in homozygous DI rats which were stained with immunoreaction products to NP and OT were more concentrated in the dorsal part of the SON and in the periphery of the PVN.
3 context sentences(design, population, supporting numbers)
Summary · context
The cellular distribution of neurophysin was examined in hypothalami and neural lobes of normal Long-Evans rats and Brattleboro rats deficient in vasopressin and a major neurophysin.
Summary · context
Tissue sections were treated with antisera to bovine, human, and rat neurophysins, using immunoperoxidase bridge techniques.
Summary · context
Antisera to oxytocin (OT) and vasopressin (VP) were applied to adjacent sections.
This result measures the body's own Oxytocin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0126151202
03LabEndogenous levelEx vivo tissueMechanism
In heterozygous Brattleboro rats, darker neurophysin-reactive cells had the same distribution as cells containing oxytocin.
In plain wordsIn lab tests of heterozygous Brattleboro rat tissue, cells positive for the body's own oxytocin were distributed like darker neurophysin-positive cells.
Peptide
Oxytocin
Outcome
Not applicableDistribution of oxytocin-containing cells in heterozygous Brattleboro rats
Study
ratn: not reported in source
hypothalamic and neural lobe tissue sections
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
The darker cells in heterozygous Brattleboro rats had the same pattern of distribution as cells which contained OT.
3 context sentences(design, population, supporting numbers)
Summary · context
The cellular distribution of neurophysin was examined in hypothalami and neural lobes of normal Long-Evans rats and Brattleboro rats deficient in vasopressin and a major neurophysin.
Summary · context
Tissue sections were treated with antisera to bovine, human, and rat neurophysins, using immunoperoxidase bridge techniques.
Summary · context
Antisera to oxytocin (OT) and vasopressin (VP) were applied to adjacent sections.
This result measures the body's own Oxytocin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0126151203
04LabEndogenous levelEx vivo tissueMechanism
In homozygous Brattleboro rats, only some neurophysin- and oxytocin-containing cells reacted with vasopressin antiserum because of slight crossreactivity with oxytocin.
In plain wordsIn lab tests of homozygous Brattleboro rat tissue, some natural oxytocin-containing cells reacted to vasopressin antiserum through slight oxytocin crossreactivity.
Peptide
Oxytocin
Outcome
Not applicableVasopressin immunoreactivity in oxytocin-containing cells of homozygous Brattleboro rats
Study
ratn: not reported in source
hypothalamic and neural lobe tissue sections
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
In homozygous DI rats, only some of those cells which contained neurophysin and OT exhibited a positive reaction with antiserum to VP due to slight reactivity with OT.
3 context sentences(design, population, supporting numbers)
Summary · context
The cellular distribution of neurophysin was examined in hypothalami and neural lobes of normal Long-Evans rats and Brattleboro rats deficient in vasopressin and a major neurophysin.
Summary · context
Tissue sections were treated with antisera to bovine, human, and rat neurophysins, using immunoperoxidase bridge techniques.
Summary · context
Antisera to oxytocin (OT) and vasopressin (VP) were applied to adjacent sections.
This result measures the body's own Oxytocin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0126151204
05LabEndogenous levelEx vivo tissueMechanism
Results in homozygous Brattleboro rats suggested that oxytocin and vasopressin with their neurophysins are produced in different neurons in both nuclei.
In plain wordsIn lab tests of homozygous Brattleboro rat tissue, results suggested different neurons produce the body's own oxytocin and vasopressin.
Peptide
Oxytocin
Outcome
Not applicableCellular production of oxytocin and vasopressin in supraoptic and paraventricular nuclei
Study
ratn: not reported in source
hypothalamic and neural lobe tissue sections
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
About half of the magnocellular neurons in the supraoptic (SON) and paraventricular (PVN) nuclei of homozygous Brattleboro rats with diabetes insipidus (DI) were devoid of immunoreactive neurophysin, OT, and VP.
2 context sentences(design, population, supporting numbers)
Summary · context
The cells in homozygous DI rats which were stained with immunoreaction products to NP and OT were more concentrated in the dorsal part of the SON and in the periphery of the PVN.
Summary · context
The results obtained in the homozygous Brattleboro rat would suggest that OT and VP and their associated neurophysins are produced in different neurons in both the SON and PVN.
This result measures the body's own Oxytocin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0126151205
06LabEndogenous levelEx vivo tissueMechanism
In normal and heterozygous Brattleboro rats, vasopressin appeared in both oxytocin-positive and oxytocin-negative neurons.
In plain wordsIn lab tests of normal and heterozygous Brattleboro rat tissue, vasopressin appeared in neurons with and without the body's own oxytocin.
Peptide
Oxytocin
Outcome
Not applicableVasopressin in oxytocin-positive and oxytocin-negative neurons
Study
ratn: not reported in source
hypothalamic and neural lobe tissue sections
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
However, in normal rats and in heterozygous Brattleboro rats, VP appeared to be present in both OT-positive and OT-negative neurons suggesting that some cells may have the capacity to synthesize two hormones.
3 context sentences(design, population, supporting numbers)
Summary · context
The cellular distribution of neurophysin was examined in hypothalami and neural lobes of normal Long-Evans rats and Brattleboro rats deficient in vasopressin and a major neurophysin.
Summary · context
Tissue sections were treated with antisera to bovine, human, and rat neurophysins, using immunoperoxidase bridge techniques.
Summary · context
Antisera to oxytocin (OT) and vasopressin (VP) were applied to adjacent sections.
This result measures the body's own Oxytocin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0126151206

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
12 Oct 2026, 11:11 UTC
Study results updated
Version 1
9 Oct 2026, 02:54 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

MeSH terms

PepBank is a research reference. Talk to a doctor before you use any peptide.