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Study · Other design · 1979

3 results

The chemistry and biology of thymosin. II. Amino acid sequence analysis of thymosin alpha1 and polypeptide beta1.

Low TL, Goldstein AL

The Journal of biological chemistry1 Feb 1979Cited by 134 (Europe PMC)

PMID762108 (opens in a new tab)DOI10.1016/s0021-9258(17)37901-2 (opens in a new tab)PepBankPUB-00762108
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Summary

The amino acid sequences of two polypeptide components of thymosin Fraction 5 termed thymosin alpha1 and polypeptide beta1 have been established. The sequences were determined by automatic Edman degradation of the intact molecules as well as by manual sequence analysis of the enzymatic cleavage products. Thymosin alpha1, an immunologically active polypeptide, is highly acidic with an isoelectric point of 4.2. This molecule is composed of 28 amino acid residues with acetylserine as the NH2 terminus. A chemically synthesized molecule of thymosin alpha1 has been found to be as active as the natural molecule in our bioassay systems. Polypeptide beta1 is a molecule consisting of 74 amino acid residues and has an isoelectric point of 6.7. This peptide is not biologically active in our assay systems, suggesting that it is not involved in thymic hormone action. The sequence of beta1 was found to be identical with ubiquitin and a portion of protein A24, a nuclear chromosomal protein. The relationships among these proteins are discussed.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study3

1 result from studies that gave the peptide. No result from this study comes from people.

  1. 01LabOther designActivity measurement

    Chemically synthesized thymosin alpha1 was as active as the natural molecule in the authors' bioassay systems.

    In plain wordsIn lab tests, synthetic thymosin alpha1 was as active as the natural molecule.

    Peptide
    Thymosin alpha-1
    Outcome
    No significant changeBioactivity of synthetic versus natural thymosin alpha1
    Study

    n: not reported in source

    Thymosin alpha1 from thymosin Fraction 5
    Comparator
    Natural thymosin alpha1
    Outcome group
    Immune response · Immune and inflammation
    Studied dose
    Dose not stated

    Source sentences

    Summary
    A chemically synthesized molecule of thymosin alpha1 has been found to be as active as the natural molecule in our bioassay systems.

Studies that did not test the peptide alone2

In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin alpha-1.

  1. 02LabNot a test of Thymosin alpha-1 aloneIn vitro (cell-free)Other

    Thymosin alpha1 is highly acidic with an isoelectric point of 4.2.

    In plain wordsIn lab tests, thymosin alpha1 was found to be highly acidic.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableIsoelectric point of thymosin alpha1
    Study

    n: not reported in source

    Thymosin alpha1 from thymosin Fraction 5
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Measurements

    Isoelectric point
    4.2 pH
    thymosin alpha1

    Source sentences

    Summary
    Thymosin alpha1, an immunologically active polypeptide, is highly acidic with an isoelectric point of 4.2.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  2. 03LabNot a test of Thymosin alpha-1 aloneIn vitro (cell-free)Other

    Thymosin alpha1 consists of 28 amino acid residues with acetylserine as the NH2 terminus.

    In plain wordsIn lab tests, thymosin alpha1 was found to have 28 amino acids and an acetylserine at one end.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableLength and N-terminus of thymosin alpha1
    Study

    n: not reported in source

    Thymosin alpha1 from thymosin Fraction 5
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Measurements

    Amino acid residues
    28 residues
    thymosin alpha1

    Source sentences

    Summary
    This molecule is composed of 28 amino acid residues with acetylserine as the NH2 terminus.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    10 Oct 2026, 08:55 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 03:02 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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