The delta EEG (sleep)-inducing peptide (DSIP). XI. Amino-acid analysis, sequence, synthesis and activity of the nonapeptide.

Schoenenberger GA, Maier PF, Tobler HJ, Wilson K, Monnier M

Pflugers Archiv : European journal of physiology1 Sept 1978Cited by 41 (Europe PMC)

PMID568769DOI10.1007/bf00581575PepBankPUB-00568769

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

4 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Activity measurement
In rabbits, intraventricular synthetic DSIP had a highly specific spindle EEG-enhancing effect.
In plain wordsIn rabbits, DSIP given into the brain ventricles increased spindle brain-wave activity.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
IncreaseEEG spindle activity
Study
rabbitn = 61
Comparator
CSF-like solution
Outcome group
Sleep · Sleep
Studied dose
6 nmol/kg · intraventricular infusion · 3.5 minAs given in this study (rabbit).
Source sentences
Summary
A highly specific delta and spindle EEG-enhancing effect of the synthetic DSIP could be demonstrated.
2 context sentences(design, population, supporting numbers)
Summary · context
All 9 synthetic peptides were infused intraventricularly in rabbits (6 nmol/kg in 0.05 ml of CSF-like solution over 3.5 min) and tested under double-blind conditions.
Summary · context
A total of 61 rabbits including controls were used.
Report a problemFND-0056876903
02AnimalAnimal study (in vivo)Activity measurement
In rabbits, intraventricular synthetic DSIP raised EEG delta activity by a mean of 35% in the neocortex and limbic cortex compared with control animals.
In plain wordsIn rabbits, DSIP given into the brain ventricles raised delta brain-wave activity by 35% compared with controls.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
IncreaseEEG delta activity versus CSF-like solution
Study
rabbitn = 61
Comparator
CSF-like solution
Outcome group
Sleep · Sleep
Studied dose
6 nmol/kg · intraventricular infusion · 3.5 minAs given in this study (rabbit).
Measurements
Mean increase of EEG delta activity
35%
neocortex and limbic cortex vs CSF-like solution
Source sentences
Summary
The mean increase of EEG delta activity reached 35% in the neocortex and limbic cortex as compared to control animals receiving CSF-like solution or any of the other 8 peptides.
2 context sentences(design, population, supporting numbers)
Summary · context
All 9 synthetic peptides were infused intraventricularly in rabbits (6 nmol/kg in 0.05 ml of CSF-like solution over 3.5 min) and tested under double-blind conditions.
Summary · context
A total of 61 rabbits including controls were used.
Report a problemFND-0056876904
03AnimalAnimal study (in vivo)Activity measurement
In rabbits, intraventricular synthetic DSIP raised EEG delta activity by a mean of 35% compared with any of the other 8 peptides.
In plain wordsIn rabbits, DSIP raised delta brain-wave activity by 35% compared with each of the other 8 peptides.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
IncreaseEEG delta activity versus 8 other peptides
Study
rabbitn = 61
Comparator
8 other synthetic peptides (metabolic products, analogues, tripeptide)
Outcome group
Sleep · Sleep
Studied dose
6 nmol/kg · intraventricular infusion · 3.5 minAs given in this study (rabbit).
Measurements
Mean increase of EEG delta activity
35%
neocortex and limbic cortex vs other 8 peptides
Source sentences
Summary
The mean increase of EEG delta activity reached 35% in the neocortex and limbic cortex as compared to control animals receiving CSF-like solution or any of the other 8 peptides.
2 context sentences(design, population, supporting numbers)
Summary · context
This compound was synthesized as well as 5 possible metabolic products (1--8, 2--9, 2--8, 1--4 and 5--9), 2 nonapeptide analogues (with one and two amino-acids exchanged) and a related tripeptide (Trp-Ser-Glu).
Summary · context
All 9 synthetic peptides were infused intraventricularly in rabbits (6 nmol/kg in 0.05 ml of CSF-like solution over 3.5 min) and tested under double-blind conditions.
Report a problemFND-0056876905
04AnimalAnimal study (in vivo)Activity measurement
In rabbits, only the pure alpha-aspartyl form of synthetic DSIP was highly active, in contrast to its beta-Asp isomer.
In plain wordsIn rabbits, only the pure alpha-aspartyl form of DSIP was highly active, unlike its beta-Asp isomer.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicableActivity of alpha-aspartyl DSIP versus beta-Asp isomer
Study
rabbitn = 61
Comparator
beta-Asp isomer
Outcome group
Sleep · Sleep
Studied dose
6 nmol/kg · intraventricular infusion · 3.5 minAs given in this study (rabbit).
Source sentences
Summary
The final chemical characterization of the synthetic DSIP revealed that only the pure alpha-aspartyl peptide is highly active in contrast to its beta-Asp isomer.
1 context sentence(design, population, supporting numbers)
Summary · context
All 9 synthetic peptides were infused intraventricularly in rabbits (6 nmol/kg in 0.05 ml of CSF-like solution over 3.5 min) and tested under double-blind conditions.
Report a problemFND-0056876906

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

05AnimalEndogenous levelAnimal study (in vivo)Other
In rabbits, a peptide that induces slow-wave EEG was isolated from dialysate of cerebral venous blood after hypnogenic stimulation of the thalamus, and later named DSIP.
In plain wordsIn rabbits, a natural sleep-linked peptide was isolated from brain blood after thalamus stimulation and named DSIP.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicableIsolation of a slow-wave EEG-inducing peptide from rabbit cerebral venous blood
Study
rabbitn: not reported in source
Outcome group
Endogenous peptide levels · Sleep
Source sentences
Summary
A peptide which induces slow-wave EEG (sleep) after intraventricular infusion into the brain has been isolated from the extracorporeal dialysate of cerebral venous blood in rabbits submitted to hypnogenic electrical stimulation of the intralaminar thalamic area.
This result measures the body's own Delta sleep-inducing peptide (DSIP). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0056876901

Studies that did not test the peptide alone1

In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Delta sleep-inducing peptide (DSIP).

06LabNot a test of Delta sleep-inducing peptide (DSIP) aloneIn vitro (cell-free)Other
Amino-acid analysis and sequence determination showed the isolated peptide to be Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu.
In plain wordsIn lab tests, the sequence of the isolated peptide was found to be nine amino acids, named DSIP.
Peptide
Delta sleep-inducing peptide (DSIP)
Outcome
Not applicableAmino acid sequence of DSIP
Study
n: not reported in source
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
It was shown by amino-acid analysis and sequence determination to be Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu and named "Delta Sleep-Inducing Peptide" (DSIP).
In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Delta sleep-inducing peptide (DSIP).
Report a problemFND-0056876902

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
9 Oct 2026, 18:17 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

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