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Study · Not a test of the peptide alone · 1977

5 results

Determination of the microscopic and macroscopic acid dissociation constants of glycyl-L-histidyl-L-lysine and related histidine peptides.

Rabenstein DL, Greenberg MS, Evans CA

Biochemistry1 Mar 1977Cited by 7 (Europe PMC)

PMID14668 (opens in a new tab)DOI10.1021/bi00624a026 (opens in a new tab)PepBankPUB-00014668
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

  1. 01LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cell-free)Other

    In molecular measurements, GHK and related histidine peptides had glycyl ammonium and histidyl imidazolium groups titrated over the same pH range.

    In plain wordsIn lab tests, GHK and related peptides had two chemical groups titrated over the same pH range.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    Not applicableGlycyl ammonium and histidyl imidazolium titration
    Study

    n: not reported in source

    proton magnetic resonance acid-base chemistry
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Source sentences

    Summary
    Chemical-shift data indicate that, at the molecular level, the glycyl ammonium and the histidyl imidazolium groups are titrated over the same pH range, with the acidity of the imidazolium group some 8 to 10 times that of the glycyl ammonium group, depending on the peptide.

    In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

  2. 02LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cell-free)Other

    In molecular measurements of GHK and related peptides, imidazolium acidity was 8 to 10 times glycyl ammonium acidity, depending on peptide.

    In plain wordsIn lab tests of GHK and related peptides, imidazolium was more acidic than glycyl ammonium, depending on peptide.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    IncreaseRelative imidazolium acidity
    Study

    n: not reported in source

    proton magnetic resonance acid-base chemistry
    Comparator
    Glycyl ammonium group
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Source sentences

    Summary
    Chemical-shift data indicate that, at the molecular level, the glycyl ammonium and the histidyl imidazolium groups are titrated over the same pH range, with the acidity of the imidazolium group some 8 to 10 times that of the glycyl ammonium group, depending on the peptide.

    In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

  3. 03LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cell-free)Other

    In molecular measurements, the GHK lysyl ammonium group was much less acidic than the other groups.

    In plain wordsIn lab tests, the GHK lysyl ammonium group was much less acidic than the other groups.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    DecreaseLysyl ammonium acidity
    Study

    n: not reported in source

    proton magnetic resonance acid-base chemistry
    Comparator
    Glycyl ammonium and histidyl imidazolium groups
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Source sentences

    Summary
    The lysyl ammonium group of Gly-His-Lys is much less acidic and is titrated over a higher pH range.
    1 context sentence
    Summary · context
    Chemical-shift data indicate that, at the molecular level, the glycyl ammonium and the histidyl imidazolium groups are titrated over the same pH range, with the acidity of the imidazolium group some 8 to 10 times that of the glycyl ammonium group, depending on the peptide.

    In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

  4. 04LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cell-free)Other

    In molecular measurements, the GHK lysyl ammonium group titrated over a higher pH range.

    In plain wordsIn lab tests, the GHK lysyl ammonium group titrated at higher pH than the glycyl ammonium and histidyl imidazolium groups.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    IncreaseLysyl ammonium titration pH
    Study

    n: not reported in source

    proton magnetic resonance acid-base chemistry
    Comparator
    Glycyl ammonium and histidyl imidazolium groups
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Source sentences

    Summary
    The lysyl ammonium group of Gly-His-Lys is much less acidic and is titrated over a higher pH range.

    In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

  5. 05LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cell-free)Other

    In molecular measurements, investigators determined microscopic and macroscopic acid-dissociation constants for GHK and related peptides from chemical shifts.

    In plain wordsIn lab tests, investigators determined GHK and related peptide acid-dissociation constants from chemical shifts.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    Not applicableAcid dissociation constant determination
    Study

    n: not reported in source

    proton magnetic resonance acid-base chemistry
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Source sentences

    Summary
    Microscopic and macroscopic acid-dissociation constants were determined from chemical-shift data for each of the peptides.

    In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    14 Oct 2026, 05:02 UTC

    Study results updated

  2. Version 1

    14 Oct 2026, 01:46 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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